Physiology Department, Faculty of Basic Medical Sciences, University of Uyo, Uyo, Akwa-Ibom State, Nigeria.
Physiology Unit, Ajayi Crowther University, Oyo, Oyo State, Nigeria.
PLoS One. 2024 Sep 6;19(9):e0308338. doi: 10.1371/journal.pone.0308338. eCollection 2024.
There is evidence that nitric oxide (NO) modulates the metabolism of glucose and lipid, and some antihypertensive medications have been shown to affect glucose and lipid metabolism. Peristrophe bivalvis is a medicinal plant that has been shown to have antihypertensive properties. The study investigated the effect of aqueous extract of Peristrophe bivalvis leaf (APB) on fasting blood glucose level (FBG) and lipid profile in rats pretreated with nitro-L-arginine methyl ester (L-NAME). Male Wistar rats (150-170 g, n=30) were randomly divided into two groups: control (CT, n=5) and L-NAME pretreated (n=25). CT received 5 mL/kg of distilled water [DW]) while L-NAME pretreated group received 60 mg/kg of L-NAME (L-NAME60) for eight weeks. After eight weeks, the L-NAME pretreated group was randomly subdivided into L-NAME group (LN), L-NAME recovery group (LRE), L-NAME ramipril group (LRA), and L-NAME APB group (LAPB). The groups received L-NAME60+DW, DW, L-NAME60+10 mg/kg ramipril, and L-NAME60+APB (200 mg/kg), respectively, for five weeks. Serum NO, lipid profile, cyclic guanosine monophosphate (cGMP), and insulin were measured by spectrophotometry, assay kits, and ELISA, respectively. Data were analysed using ANOVA at p < 0.05. At the eighth week, a fall in FBG and an increase in triglyceride, total cholesterol, and low-density lipoprotein cholesterol were recorded in L8 compared to CT. The same effects were also noticed in the thirteenth week in LN. However, FBG was significantly increased and lipid levels were decreased in LAPB compared to LN. A significant increase was observed in cGMP level in LAPB compared to LN. The study showed that APB corrected the hyperlipidemia and hypoglycemia caused by L-NAME, and this effect might be via the activation of cGMP.
有证据表明一氧化氮（NO）可调节葡萄糖和脂质的代谢，一些降压药物已被证明可影响葡萄糖和脂质代谢。蕺菜是一种具有降压特性的药用植物。本研究探讨了蕺菜叶水提物（APB）对预先给予硝基-L-精氨酸甲酯（L-NAME）的大鼠空腹血糖水平（FBG）和血脂谱的影响。雄性 Wistar 大鼠（150-170g，n=30）随机分为两组：对照组（CT，n=5）和 L-NAME 预处理组（n=25）。CT 给予 5mL/kg 蒸馏水[DW]，而 L-NAME 预处理组给予 60mg/kg L-NAME（L-NAME60），共 8 周。8 周后，将 L-NAME 预处理组随机分为 L-NAME 组（LN）、L-NAME 恢复组（LRE）、L-NAME 雷米普利组（LRA）和 L-NAME APB 组（LAPB）。各组分别给予 L-NAME60+DW、DW、L-NAME60+10mg/kg 雷米普利和 L-NAME60+APB（200mg/kg），共 5 周。用分光光度法、试剂盒和 ELISA 分别测定血清 NO、血脂谱、环鸟苷酸（cGMP）和胰岛素。数据采用方差分析，p<0.05。第 8 周时，与 CT 相比，L8 组 FBG 降低，三酰甘油、总胆固醇和低密度脂蛋白胆固醇升高。LN 组在第 13 周也观察到了同样的效果。然而，与 LN 相比，LAPB 组的 FBG 显著升高，血脂水平降低。与 LN 相比，LAPB 组 cGMP 水平显著升高。该研究表明，APB 纠正了 L-NAME 引起的高脂血症和低血糖，这种作用可能是通过激活 cGMP 实现的。
