Purchase H G, Okazaki W, Vogt P K, Hanafusa H, Burmester B R, Crittenden L B
Infect Immun. 1977 Feb;15(2):423-8. doi: 10.1128/iai.15.2.423-428.1977.
Leukosis viruses of seven subgroups were tested for oncogenicity in chickens susceptible to virus infection and to development of lymphoid leukosis (LL) tumors. All subgroup A viruses and the subgroup B virus tested produced a high incidence of LL and other related neoplasms. Viruses of subgroup C and RAV-61 of subgroup F produced a low level of LL. The RAV-50 of subgroup D produced osteopetrosis. In these tests, the viruses of subgroup E and G and one virus of subgroup F were not pathogenic, possibly because infection was not established in the chickens, the chickens were not susceptible to tumor development by these viruses, or the viruses lacked oncogenicity. All temperature-sensitive mutants of Rous sarcoma virus produced sarcomas, but the level varied. One nontransforming mutant produced sarcomas, and the other three tested produced LL. All three mutants that cause cells to grow as colonies in agar produced a high incidence of sarcomas. Thus, sarcoma viruses, by back-mutation, may lose the ability to transform cells in vitro, to make cells grow in agar colonies, or to induce sarcomas in vivo, yet they retain the ability to produce LL. Conversely, it was previously shown that leukosis viruses may be changed into viruses that transform cells in vitro and produce sarcomas in vivo by suitable passage in chicks.
对七个亚组的白血病病毒进行了致癌性测试，所用鸡对病毒感染和淋巴细胞白血病（LL）肿瘤的发生敏感。所有测试的A亚组病毒和B亚组病毒都导致LL和其他相关肿瘤的高发病率。C亚组病毒和F亚组的RAV-61导致LL的发生率较低。D亚组的RAV-50导致骨硬化症。在这些测试中，E亚组和G亚组的病毒以及F亚组的一种病毒没有致病性，可能是因为在鸡中未建立感染，鸡对这些病毒诱导的肿瘤发生不敏感，或者这些病毒缺乏致癌性。劳氏肉瘤病毒的所有温度敏感突变体都产生肉瘤，但水平有所不同。一个非转化突变体产生肉瘤，另外三个测试的突变体产生LL。所有三个能使细胞在琼脂中形成集落生长的突变体都导致肉瘤的高发病率。因此，肉瘤病毒通过回复突变可能会失去在体外转化细胞、使细胞在琼脂集落中生长或在体内诱导肉瘤的能力，但它们仍保留产生LL的能力。相反，先前已表明，白血病病毒通过在雏鸡中适当传代，可能会变成在体外转化细胞并在体内产生肉瘤的病毒。
