Iwamoto M, Uchino K, Toukairin T, Kawaguchi K, Tatebayashi T, Ogawara H, Tonosaki Y
Central Laboratory, Nippon Flour Mills Co., Ltd., Kanagawa, Japan.
Chem Pharm Bull (Tokyo). 1991 May;39(5):1323-4. doi: 10.1248/cpb.39.1323.
New 5'-nucleotidase inhibitors named NF-86I, NF-86II were recently isolated from the seeds of Areca catechu L. NF-86I and NF86II showed inhibitory effects on the growth of Streptococcus mutans MT8148(c) and Streptococcus mutans MT6715(g), respectively. In addition, these inhibitors could inhibit insoluble glucan formation from sucrose. NF-86I and NF-86II were found to be polyphenolic substances. Some polyphenols such as tannic acid bind non-specifically to proteins (tannic activity). The 5'-nucleotidase inhibitors that we isolated did not show any such activity. However, the growth inhibitory activity and the inhibitory effect on water-insoluble glucan production were equal to tannic acid. It is therefore considered that these inhibitors bind specifically to the bacterial cell surface. Our findings suggest that the 5'-nucleotidase inhibitors NF-86I and NF-86II may be useful anti-plaque preventing agents.
新型5'-核苷酸酶抑制剂NF-86I和NF-86II最近从槟榔种子中分离得到。NF-86I和NF-86II分别对变形链球菌MT8148(c)和变形链球菌MT6715(g)的生长具有抑制作用。此外，这些抑制剂能够抑制由蔗糖形成不溶性葡聚糖。已发现NF-86I和NF-86II是多酚类物质。一些多酚如鞣酸可与蛋白质非特异性结合（鞣酸活性）。我们分离得到的5'-核苷酸酶抑制剂未表现出任何此类活性。然而，其生长抑制活性和对水不溶性葡聚糖产生的抑制作用与鞣酸相当。因此，认为这些抑制剂可特异性结合细菌细胞表面。我们的研究结果表明，5'-核苷酸酶抑制剂NF-86I和NF-86II可能是有用的防菌斑剂。
