Dong Jian, Solntsev Kyril M, Tolbert Laren M
School of Chemistry and Biochemistry, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, Georgia 30332-0400, USA.
J Am Chem Soc. 2009 Jan 21;131(2):662-70. doi: 10.1021/ja806962e.
O-Alkyl synthetic analogues of the green fluorescent protein chromophore are nonfluorescent in solution but demonstrate a bright emission in the crystalline state. Three-dimensional steady-state and time-resolved emission spectroscopies in the solid state, as well as single-crystal X-ray diffraction, reveal the nature of complex emission in the crystals. A hypsochromic emission shift with an increase of the alkyl size is determined by the monomer-aggregate emission ratio.
绿色荧光蛋白发色团的O-烷基合成类似物在溶液中无荧光，但在晶体状态下呈现明亮发射。固态的三维稳态和时间分辨发射光谱以及单晶X射线衍射揭示了晶体中复杂发射的本质。随着烷基尺寸增加的蓝移发射由单体-聚集体发射比率决定。
