Gardner M B, Brown J C, Charman H P, Stephenson J R, Rongey R W, Hauser D E, Diegmann F, Howard E, Dworsky R, Gilden R V, Huebner R J
Int J Cancer. 1977 Apr 15;19(4):581-9. doi: 10.1002/ijc.2910190420.
We investigated the reliability of the fixed cell indirect fluorescent antibody (IFA) peripheral blood smear test as an index of systemic infection with FeLV. Positive results with this test were found to correlate well with detectable FeLV p30 antigen in bone-marrow smears by IFA, in serum and tissue by competition immunoassays, and with type-C particles in bone marrow or spleen by electron microscopy. Most cats with lymphoma, anemia or infectious peritonitis were positive for FeLV and showed a poor or absent antibody response to FeLV p30, gp70, and FOCMA antigens. Most older cats with lymphoma, carcinoma, or sarcoma were negative for FeLV expression and also lacked these FeLV-related antibodies. Detectable immunologic response to FeLV p30 and gp70 proteins and a high-titered FOCMA antibody response were generally restricted to certain healthy cats exposed to FeLV. Antibody to endogenous RD-114 viral p30 and gp70 was not detected in any of a large number of feline sera tested. The prevalence of FeLV-related diseases and immunologic responses to FeLV in healthy cats was directly correlated with the degree of FeLV exposure. By using the IFA blood smear and FOCMA antibody tests one can monitor the horizontal spread of FeLV in multi-cat household and accurately predict the FeLV disease susceptibilty or resistance of individual cats in each environments.
我们研究了固定细胞间接荧光抗体（IFA）外周血涂片检测作为猫白血病病毒（FeLV）全身感染指标的可靠性。结果发现，该检测的阳性结果与通过IFA在骨髓涂片中、通过竞争免疫测定法在血清和组织中检测到的FeLV p30抗原，以及通过电子显微镜在骨髓或脾脏中检测到的C型颗粒密切相关。大多数患有淋巴瘤、贫血或传染性腹膜炎的猫FeLV呈阳性，并且对FeLV p30、gp70和FOCMA抗原的抗体反应较弱或缺失。大多数患有淋巴瘤、癌或肉瘤的老年猫FeLV表达呈阴性，也缺乏这些与FeLV相关的抗体。对FeLV p30和gp70蛋白可检测到的免疫反应以及高滴度的FOCMA抗体反应通常仅限于某些接触过FeLV的健康猫。在大量检测的猫血清中，均未检测到针对内源性RD - 114病毒p30和gp70的抗体。健康猫中FeLV相关疾病的患病率以及对FeLV的免疫反应与FeLV暴露程度直接相关。通过使用IFA血涂片和FOCMA抗体检测，可以监测FeLV在多猫家庭中的水平传播，并准确预测每个环境中个体猫对FeLV疾病的易感性或抵抗力。
