Goussard J, Lechevrel C, Roussel G, Cren H, Bera O, Sala M
Laboratoire d'Analyses Isotopiques, Centre François Baclesse, Caen, France.
Clin Chem. 1991 Oct;37(10 Pt 1):1759-62.
We studied the ELSA-pS2 immunoradiometric kit (CIS Bio International) for pS2 protein assay in breast cancer cytosols according to classic validation methods. In addition, we studied correlations between pS2, steroid receptors, and cathepsin-D assays. Repeatability (CV = 1.5% to 4.8%) and reproducibility (CV = 1.6% to 4.9%) were good. The results were linearly related to pS2 concentrations between 205 and 2200 ng/L; the detection limit was 40 ng/L. The accuracy of the assay was measured by assessing recovery; analytical recoveries were near 100% throughout the standard curve. The use of different compounds for cytosol preparation (Tris 10 mmol/L or phosphate 25 mmol/L, KCl 0.4 mol/L, bovine serum albumin 1 g/L) had no effect on pS2 results. pS2 was assayed in breast tumor cytosols from 197 postmenopausal and 92 premenopausal patients. The mean value was 24 micrograms/g of protein; the median and 25th and 75th percentiles were 6, 1, and 23 micrograms/g protein, respectively. We observed a relation between concentrations of pS2 and those of estrogen and progesterone receptors, but there was no relationship between the concentrations of pS2 and cathepsin-D.
我们根据经典验证方法，研究了用于检测乳腺癌细胞溶质中pS2蛋白的ELSA-pS2免疫放射分析试剂盒（CIS Bio International公司）。此外，我们还研究了pS2、类固醇受体和组织蛋白酶D检测之间的相关性。重复性（变异系数CV = 1.5%至4.8%）和再现性（CV = 1.6%至4.9%）良好。结果与205至2200 ng/L之间的pS2浓度呈线性相关；检测限为40 ng/L。通过评估回收率来测定该分析方法的准确性；在整个标准曲线范围内，分析回收率接近100%。使用不同化合物制备细胞溶质（10 mmol/L Tris或25 mmol/L磷酸盐、0.4 mol/L KCl、1 g/L牛血清白蛋白）对pS2检测结果无影响。对197例绝经后和92例绝经前患者的乳腺肿瘤细胞溶质进行了pS2检测。平均值为24微克/克蛋白质；中位数以及第25和第75百分位数分别为6、1和23微克/克蛋白质。我们观察到pS2浓度与雌激素和孕激素受体浓度之间存在关联，但pS2浓度与组织蛋白酶D浓度之间无关联。
