Tomasoni A, Scanziani E, Massazza G, Giudici G, Sironi G, Caslini C, Rambaldi A, Giavazzi R
Istituto di Ricerche Farmacologiche Mario Negri, Bergamo, Italy.
Clin Exp Metastasis. 1991 Sep-Oct;9(5):485-97. doi: 10.1007/BF01785533.
The MOC-25 tumour arose spontaneously in a female nude mouse and was established as a continuous line intraperitoneally in nude mice, where it reproduces the topological features of its origin, growing preferentially in the uterus, ovaries and liver. Karyotype analysis showed that MOC-25 cells are hyperdiploid. Tumorigenicity and malignant behaviour were studied by transplanting tumour cells into different sites in nude mice. The comparison of tumour take after i.p. and s.c. injections of scaled concentrations of MOC-25 cell suspension showed preferential growth in the peritoneum. Regardless of the route of implantation (s.c., i.v., i.p.), this tumour rapidly and preferentially disseminated to the liver, uterus, ovaries, spleen and bone marrow. No significant differences in tumour growth and metastatic behaviour were observed when MOC-25 was injected in ovariectomized nude mice or in male nude mice. Morphology studies using light and electron microscopy, immunophenotyping and molecular analysis indicated a B-lymphoid origin of the MOC-25 tumour.
MOC - 25肿瘤自发产生于一只雌性裸鼠，随后在裸鼠体内通过腹腔注射建立起连续传代细胞系，在该细胞系中它重现了其起源的拓扑学特征，优先在子宫、卵巢和肝脏中生长。核型分析表明MOC - 25细胞为超二倍体。通过将肿瘤细胞移植到裸鼠的不同部位来研究其致瘤性和恶性行为。腹腔内和皮下注射不同浓度的MOC - 25细胞悬液后对肿瘤形成情况的比较显示，肿瘤在腹膜中优先生长。无论植入途径如何（皮下、静脉内、腹腔内），该肿瘤都会迅速且优先地扩散至肝脏、子宫、卵巢、脾脏和骨髓。当将MOC - 25注射到去卵巢裸鼠或雄性裸鼠体内时，未观察到肿瘤生长和转移行为有显著差异。使用光学显微镜和电子显微镜进行的形态学研究、免疫表型分析和分子分析表明MOC - 25肿瘤起源于B淋巴细胞。
