Chai B F
Shanghai Institute of Traumatology and Orthopaedics.
Zhonghua Wai Ke Za Zhi. 1991 May;29(5):305-7, 334-5.
The patients with late osteoarthritis of the knee joint were given oral tetracycline to label the newly formed bone tissues before they were subjected to high tibial osteotomies or knee replacements. During the operation, the sclerotic subchondral bone tissue of the tibia and/or femur were procured. The bone specimens were processed into undecalcified sections and studied under fluorescence microscope. Apart from the autofluorescence of the trabeculae, strips of golden green labelled fluorescence of high intensity were noted along their peripheries, indicating that there were formatting and deposition of new bone along the periphery of the trabeculae. These new bone tissues were produced by the osteoblasts abutting the trabecular periphery. In the fatty tissues of the marrow interstices between the trabeculae, scattered dots and small flakes of golden green labelled fluorescence emerged at first. Then the labelled-fluorescent dots and flakes agglomerated into large pieces of more intense fluorescence with concomitant decrease in fatty tissues. Eventually, these large pieces of labelled fluorescence fused with those of the trabeculae and there was a further decrease in fatty tissues. These findings suggested that the formation and deposition of new bone tissues in the marrow interstices proceeded according to above three stages. The new bone tissues were produced by marrow stromal cells. The two kinds of new bone tissues increased the bone mass and rendered sclerotic changes in upper end of the tibia and lower end of the femur in osteoarthritis of knee joint.
膝关节晚期骨关节炎患者在接受高位胫骨截骨术或膝关节置换术前，口服四环素以标记新形成的骨组织。手术过程中，获取胫骨和/或股骨的硬化软骨下骨组织。将骨标本制成不脱钙切片，在荧光显微镜下进行研究。除了小梁的自发荧光外，沿其周边可见高强度的金绿色标记荧光条带，表明沿小梁周边有新骨形成和沉积。这些新骨组织由紧靠小梁周边的成骨细胞产生。在小梁间骨髓间隙的脂肪组织中，起初出现散在的金绿色标记荧光点和小薄片。然后，标记荧光点和薄片聚集成荧光更强的大片，同时脂肪组织减少。最终，这些大片标记荧光与小梁的荧光融合，脂肪组织进一步减少。这些发现提示骨髓间隙中新骨组织的形成和沉积按上述三个阶段进行。新骨组织由骨髓基质细胞产生。这两种新骨组织增加了骨量，并使膝关节骨关节炎患者胫骨上端和股骨下端出现硬化改变。
