Daneshvar Hamid, Molaei Mohammad M, Afshar Reza Malekpour, Kamiabi Hosein, Burchmore Richard, Hagan Paul, Phillips R Stephen
Medical School, University of Kerman, Kerman, Iran.
Vet Immunol Immunopathol. 2009 May 15;129(1-2):28-35. doi: 10.1016/j.vetimm.2008.12.002. Epub 2008 Dec 6.
The clinicopathological changes following infection with an attenuated line of Leishmania infantum (L. infantum H-line) were evaluated in mixed breed dogs. Two groups of dogs were infected intravenously (i.v.) or intradermally (i.d.) with L. infantum H-line and two control groups were infected i.v. or i.d. with L. infantum wild-type (L. infantum WT). None of the dogs, which were infected i.v. or i.d. with L. infantum H-line, showed any abnormalities during the observation period. In contrast, two out of three dogs, which were infected i.v. with L. infantum WT, developed clinical signs of disease. In addition, no histopathological changes were seen in the liver and spleen of the dogs infected with the attenuated line of parasite, whereas the histopathological changes in the two dogs infected i.v. with L. infantum WT were severe in form and manifested by infiltration of high numbers of inflammatory cells. No promastigotes were found in cultures set up from spleens and livers of dogs infected with L. infantum H-line at 12 months post-infection, whereas promastigotes were seen in the spleen and liver cultures from 2 dogs infected i.v. with L. infantum WT. Serum levels of total IgG anti-Leishmania antibody were raised in all dogs. The antibody level in the serum of dogs infected i.v. with L. infantum WT was higher than that in dogs infected with L. infantum H-line. These results show no clinicopathological abnormalities in the dogs infected with gentamicin-attenuated L. infantum H-line. Moreover, L. infantum H-line induced IgG anti-Leishmania antibody in the dogs.
在杂种犬中评估了感染婴儿利什曼原虫减毒株（婴儿利什曼原虫H株）后的临床病理变化。将两组犬分别通过静脉注射（i.v.）或皮内注射（i.d.）感染婴儿利什曼原虫H株，另外两组对照组分别通过静脉注射或皮内注射感染婴儿利什曼原虫野生型（婴儿利什曼原虫WT）。通过静脉注射或皮内注射感染婴儿利什曼原虫H株的犬在观察期内均未表现出任何异常。相比之下，通过静脉注射感染婴儿利什曼原虫WT的三只犬中有两只出现了疾病的临床症状。此外，感染减毒寄生虫株的犬的肝脏和脾脏未观察到组织病理学变化，而通过静脉注射感染婴儿利什曼原虫WT的两只犬的组织病理学变化严重，表现为大量炎性细胞浸润。在感染婴儿利什曼原虫H株的犬感染后12个月从脾脏和肝脏建立的培养物中未发现前鞭毛体，而在通过静脉注射感染婴儿利什曼原虫WT的两只犬的脾脏和肝脏培养物中可见前鞭毛体。所有犬的血清总抗利什曼原虫IgG抗体水平均升高。通过静脉注射感染婴儿利什曼原虫WT的犬血清中的抗体水平高于感染婴儿利什曼原虫H株的犬。这些结果表明，感染庆大霉素减毒的婴儿利什曼原虫H株的犬没有临床病理异常。此外，婴儿利什曼原虫H株在犬中诱导产生了抗利什曼原虫IgG抗体。
