Sellyei Boglárka, Varga Zsuzsanna, Ivanics Eva, Magyar Tibor
Veterinary Medical Research Institute of the Hungarian Academy of Sciences, H-1581 Budapest, P.O. Box 18, Hungary.
Acta Vet Hung. 2008 Dec;56(4):429-40. doi: 10.1556/AVet.56.2008.4.1.
Sixty-one avian strains of Pasteurella multocida were characterised and compared by biochemical tests, capsular PCR typing and ERIC-PCR. The strains were recovered from various avian species (goose, duck, Muscovy duck, turkey, chicken and pheasant) and represented different geographic locations in Hungary. Forty-two strains (69%) were identified as P. multocida subsp. multocida and 19 strains (31%) as P. multocida subsp. septica. The strains were grouped into 7 different biovars (1, 2, 3, 4, 5, 6 and 7). The most prevalent biovars were 1 (25%), 3 (21%) and 6 (21%). Most of the duck isolates (90%) belonged to biovar 1 or 6. The most frequent capsular type was A (93.5%). Type F represented only a small number (6.5%) of the strains. Other capsular types were not identified. From the 61 isolates 24 different fingerprint patterns were generated by ERIC-PCR assay. Based on cluster analysis the strains could be grouped into four larger and four mini-clusters that showed considerable correlation with the geographical origin and the host species. The results indicate that ERIC-PCR may be a suitable technique for studying the host adaptation of P. multocida and the epidemiology of fowl cholera.
通过生化试验、荚膜PCR分型和ERIC-PCR对61株禽多杀性巴氏杆菌进行了特性鉴定和比较。这些菌株从不同禽类（鹅、鸭、番鸭、火鸡、鸡和雉鸡）中分离得到，代表了匈牙利不同的地理位置。42株（69%）被鉴定为多杀性巴氏杆菌多杀亚种，19株（31%）为多杀性巴氏杆菌败血亚种。这些菌株被分为7个不同的生物型（1、2、3、4、5、6和7）。最常见的生物型是1型（25%）、3型（21%）和6型（21%）。大多数鸭分离株（90%）属于生物型1或6。最常见的荚膜型是A（93.5%）。F型仅占少数菌株（6.5%）。未鉴定出其他荚膜型。通过ERIC-PCR分析，从61株分离株中产生了24种不同的指纹图谱。基于聚类分析，这些菌株可分为四个较大的簇和四个较小的簇，它们与地理来源和宿主物种显示出相当的相关性。结果表明，ERIC-PCR可能是研究多杀性巴氏杆菌宿主适应性和禽霍乱流行病学的一种合适技术。
