Petersen Kamille D, Christensen Henrik, Bisgaard Magne, Olsen John E
Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, 4 Stigbøljen, DK-1870 Frederiksberg C, Copenhagen, Denmark1.
Microbiology (Reading). 2001 Oct;147(Pt 10):2739-2748. doi: 10.1099/00221287-147-10-2739.
The genetic diversity of Pasteurella multocida, the aetiological agent of fowl cholera, was investigated. The strain collection comprised 69 clinical isolates representing a wide spectrum of hosts and geographic origin. The three type strains for the subspecies of P. multocida were also included. Avian isolates of P. multocida subsp. multocida and P. multocida subsp. septica did not represent separate lines by HpaII ribotyping and the two type strains of mammalian origin (porcine and cat bite) seemed to be representative of avian strains of P. multocida subspp. multocida and septica. By ribotyping, all P. multocida subsp. gallicida strains, except one chicken isolate and the type strain, clustered together. This indicated that the bovine type strain was not representative of this subspecies and that most strains of P. multocida subsp. gallicida are genetically related and may be distantly related to other P. multocida isolates, including those of avian origin. By 16S rRNA and atpD sequence comparisons of selected strains, including both P. multocida isolated from birds and mammals and selected distantly related Pasteurella species associated with birds and mammals, it was found that P. multocida is monophyletic. Extended DNA-DNA hybridizations are highly indicated since strains may exist which would connect the existing subspecies at species level. The considerable genetic diversity of P. multocida fowl cholera isolates is probably related to the clonal nature of this organism, resulting in many divergent lines.
对禽霍乱的病原体多杀性巴氏杆菌的遗传多样性进行了研究。菌株收集包括69株临床分离株，代表了广泛的宿主和地理来源。还包括多杀性巴氏杆菌亚种的三株模式菌株。多杀性巴氏杆菌亚种多杀性亚种和多杀性巴氏杆菌亚种败血亚种的禽分离株通过HpaII核糖体分型不代表单独的谱系，两株哺乳动物来源（猪和猫咬伤）的模式菌株似乎代表了多杀性巴氏杆菌亚种多杀性亚种和败血亚种的禽菌株。通过核糖体分型，除一株鸡分离株和模式菌株外，所有多杀性巴氏杆菌亚种鸡亚种菌株聚集在一起。这表明牛模式菌株不代表该亚种，并且多杀性巴氏杆菌亚种鸡亚种的大多数菌株在遗传上相关，并且可能与其他多杀性巴氏杆菌分离株（包括禽源分离株）有较远的亲缘关系。通过对选定菌株的16S rRNA和atpD序列比较，包括从鸟类和哺乳动物分离的多杀性巴氏杆菌以及与鸟类和哺乳动物相关的选定远缘巴氏杆菌物种，发现多杀性巴氏杆菌是单系的。由于可能存在在物种水平上连接现有亚种的菌株，因此强烈建议进行扩展的DNA-DNA杂交。多杀性巴氏杆菌禽霍乱分离株的相当大的遗传多样性可能与其克隆性质有关，导致许多不同的谱系。
