[成簇规律间隔短回文重复序列：结构、功能及应用——综述]

[Clustered regularly interspaced short palindromic repeats: structure, function and application--a review].

作者信息

Cui Yujun, Li Yanjun, Yan Yanfeng, Yang Ruifu

机构信息

State Key laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.

出版信息

Wei Sheng Wu Xue Bao. 2008 Nov;48(11):1549-55.

PMID:19149174

Abstract

CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats), the basis of spoligotyping technology, can provide prokaryotes with heritable adaptive immunity against phages' invasion. Studies on CRISPR loci and their associated elements, including various CAS (CRISPR-associated) proteins and leader sequences, are still in its infant period. We introduce the brief history', structure, function, bioinformatics research and application of this amazing immunity system in prokaryotic organism for inspiring more scientists to find their interest in this developing topic.

摘要

CRISPRs（成簇规律间隔短回文重复序列）是间隔寡核苷酸分型技术的基础，可为原核生物提供针对噬菌体入侵的可遗传适应性免疫。对CRISPR位点及其相关元件的研究，包括各种CAS（CRISPR相关）蛋白和前导序列，仍处于起步阶段。我们介绍了这个惊人的原核生物免疫系统的简史、结构、功能、生物信息学研究及应用，以激励更多科学家对这个正在发展的领域产生兴趣。