Sun Yuan, Qi Qiaofen, Liang Bingbing, Cheng Dan, Li Na, Yu Moyang, Wang Yufei, Liu Nihong, Zhu Qinghu, Qiu Huaji
Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 15000, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Oct;24(10):1734-9.
Classical swine fever (CSF), which is caused by classical swine fever virus (CSFV), causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. In this study, a recombinant replication-defective human adenovirus expressing the CSFV E2 gene (rAdV-E2) was generated and evaluated for the immunogenicity in rabbits. The results showed that the rabbits immunized with rAdV-E2 developed high-level CSFV-specific antibodies. The rAdV-E2-immunized rabbits were all free of the regular fever and the viral replication in the spleen upon challenge with C-strain, which were seen in the rabbits immunized with the parent adenovirus of rAdV-E2. This indicates that the recombinant adenovirus can be an attractive candidate vaccine against CSF.
经典猪瘟（CSF）由经典猪瘟病毒（CSFV）引起，在亚洲和欧洲的许多国家给养猪业造成了重大损失。CSFV的E2糖蛋白是中和抗体的主要靶标。在本研究中，构建了一种表达CSFV E2基因的重组复制缺陷型人腺病毒（rAdV-E2），并评估了其在兔体内的免疫原性。结果显示，用rAdV-E2免疫的兔产生了高水平的CSFV特异性抗体。用rAdV-E2免疫的兔在受到C株攻击后均未出现正常发热，脾脏中也未出现病毒复制，而在使用rAdV-E2亲本腺病毒免疫的兔中则出现了这些情况。这表明重组腺病毒可能是一种有吸引力的抗CSF候选疫苗。
