Division of Swine Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
Res Vet Sci. 2010 Feb;88(1):77-82. doi: 10.1016/j.rvsc.2009.06.005. Epub 2009 Jul 7.
Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), which causes significant economic losses to the pig industry worldwide. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. This study was aimed to develop a recombinant human adenovirus type 5 expressing the CSFV E2 gene (rAdV-E2) and evaluate its efficacy in rabbits and pigs. The results showed that the rabbits and the pigs immunized with the rAdV-E2 developed high-level CSFV-specific neutralizing antibodies. The rAdV-E2-immunized rabbits were protected from fever induced by infection with C-strain, which is pathogenic to the rabbit, and the rAdV-E2-immunized pigs were protected from lethal challenge with highly virulent Shimen strain. This indicates that the recombinant adenovirus can be an attractive candidate vaccine for preventing CSF.
经典猪瘟病毒（CSFV）是引起经典猪瘟（CSF）的病原体，它给全球的养猪业造成了巨大的经济损失。CSFV 的 E2 糖蛋白是中和抗体的主要靶标。本研究旨在开发一种表达 CSFV E2 基因的重组人 5 型腺病毒（rAdV-E2），并评估其在兔和猪中的功效。结果表明，用 rAdV-E2 免疫的兔和猪产生了高水平的 CSFV 特异性中和抗体。rAdV-E2 免疫的兔可免受 C 株感染引起的发热（C 株对兔具有致病性），rAdV-E2 免疫的猪可免受高致病性石门株的致死性攻击。这表明重组腺病毒可能是预防 CSF 的一种有吸引力的候选疫苗。
