[Primary study on suspension cell culture and polysaccharides content of Achyranthes bidentata].

OBJECTIVE

To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.

METHOD

The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.

RESULT AND CONCLUSION

The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).