Xue Jian-Ping, Shi Jing-Ying
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China.
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2467-9.
To study the relationship between the condition of cell suspension culture and the polysaccharides content in Achyranthes bidentata.
The methods of orthogonal test and alone factor test were used to study the effect of culture time, inoculation concentration, carbon source and pH.
The results showed that the optimum medium to induce polysaccharides in A. bidentata was 1/4MS + 6-BA 0.5 + 2,4-D 1.0 mg x L(-1) + sucrose 2% + glucose 2% + fructose 1% cultured after 50 d. The suitable pH was 6.0, and the optimum inoculation concentration was 7g x L(-1).
