Li Ming-Jun, Li Ping, Zhang Xiao-Li, Zhao Xi-Ting
College of Life Science, Henan Normal University, Xinxiang 453007, China.
Zhongguo Zhong Yao Za Zhi. 2008 Apr;33(7):745-9.
To study the effect of medium components on the callus induction and the contents of polysaccharides in calli from Achyranthes bidentata.
Leaves and stems were selected as explants. The effects of six kinds of factors including basal culture medium, carbon source, 2,4-D, 6-BA, TDZ, CH on the callus induction and the contents of ABPS in calli on the high growth point were studied by orthogonal design method. The data were analyzed with range analysis and variance analysis.
To leaf, the optimal medium of callus induction was B5 with 2 mg x L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 1 g x L(-1) CH; to stem, the optimal one was B5 with2 mg x L(-1) 2,4-D, 1 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 0.5 g x L(-1) CH. In order to obtain higher contents ABPS, to leaf calli, the optimal medium was LS with 1 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) sucrose; to stem calli, the optimal one was LS with 1 mg L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) glucose.
The optimal media of callus induction were established with stems and leaves of A. bidentata as explants and with a view to an industrial production of polysaccharides by tissue and cell culture.
研究培养基成分对牛膝愈伤组织诱导及愈伤组织中多糖含量的影响。
选取叶片和茎段作为外植体。采用正交设计法研究基础培养基、碳源、2,4-D、6-BA、TDZ、CH六种因素对牛膝高生长点愈伤组织诱导及愈伤组织中牛膝多糖（ABPS）含量的影响。采用极差分析和方差分析对数据进行分析。
对于叶片，愈伤组织诱导的最佳培养基为B5培养基，添加2 mg·L⁻¹ 2,4-D、0.5 mg·L⁻¹ 6-BA、30 g·L⁻¹葡萄糖和1 g·L⁻¹ CH；对于茎段，最佳培养基为B5培养基，添加2 mg·L⁻¹ 2,4-D, 1 mg·L⁻¹ 6-BA、30 g·L⁻¹葡萄糖和0.5 g·L⁻¹ CH。为了获得较高含量的ABPS，对于叶片愈伤组织，最佳培养基为LS培养基，添加1 mg·L⁻¹ 6-BA, 1 mg·L⁻¹ TDZ和30 g·L⁻¹蔗糖；对于茎段愈伤组织，最佳培养基为LS培养基，添加1 mg·L⁻¹ 2,4-D、0.5 mg·L⁻¹ 6-BA、1 mg·L⁻¹ TDZ和30 g·L⁻¹葡萄糖。
以牛膝的茎和叶为外植体，建立了愈伤组织诱导的最佳培养基，以期通过组织和细胞培养实现多糖的工业化生产。
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