[红花水提取物对氧化型低密度脂蛋白诱导的大鼠心脏微血管内皮细胞损伤的电子自旋共振研究及保护作用]

[ESR study and protection of water extract of Carthamus tinctorius on ox-LDL induced injury in rat cardiac microvascular endothelial cell].

作者信息

Ye Jin-Xia, Liang Ri-Xin, Wang Lan, Yang Bin, An Rong-Shu

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2513-7.

PMID:19149262

Abstract

OBJECTIVE

To observe the antioxidant effects of water extract of Carthamus tinctorius on ox-LDL induced injury in rat cardiac microvascular endothelial cell and detecting oxygen derived free radicals (OFR) to explore the antioxidant mechanisms.

METHOD

By using the third generation of rat cardiac microvascular endothelial cells (rCMEC), the protection of water extract of C. tinctorius was investigated after ox-LDL (100 mg x L(-1) induced damage. The supernatant was collected for detecting lactate dehydrogenase (LDH), malondialdehyde (MDA), superoxide dismutase (SOD), xanthine oxidase (XOD), glutathione peroxidase (GSH-Px), nitric oxide (NO), nitric oxide synthase (NOS) activity, and cell suspension was collected for detecting reactive oxygen species (ROS) by electron spin resonance (ESR).

RESULT

Water extract of C. tinctorius increased the rCMEC survival rate, reduced LDH, MDA and XOD levels, and improved SOD, GSH-Px and NOS activity, while in the cell suspension ROS signal decreased significantly.

CONCLUSION

Water extract of C. tinctorius has antioxidation. The mechanisms are likely related with scavenging of free radicals, enhancing its clearance, enhancing endogenous antioxidant activity.

摘要

目的

观察红花水提取物对氧化型低密度脂蛋白（ox-LDL）诱导的大鼠心脏微血管内皮细胞损伤的抗氧化作用，并检测氧自由基（OFR）以探讨其抗氧化机制。

方法

采用第三代大鼠心脏微血管内皮细胞（rCMEC），观察在ox-LDL（100 mg·L⁻¹）诱导损伤后红花水提取物的保护作用。收集上清液检测乳酸脱氢酶（LDH）、丙二醛（MDA）、超氧化物歧化酶（SOD）、黄嘌呤氧化酶（XOD）、谷胱甘肽过氧化物酶（GSH-Px）、一氧化氮（NO）、一氧化氮合酶（NOS）活性，收集细胞悬液用电子自旋共振（ESR）检测活性氧（ROS）。