Chen Che, Dong Jian-yong, Liu Kai, Liu Yong-qi, Wang Ya-li
Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhong Yao Cai. 2007 Sep;30(9):1099-102.
To investigate protective effect of total flavonoids of Hedysarum polybotry on human umbilical vein endothelial cells injury induced by hydrogen peroxide.
Chemical light assay was used to detect the oxygen radicals scavenging capacity of total flavonoids of Hedysarum polybotry. Lactate dehydrogenase (LDH) in the culture medium, malondialdelyde (MDA) and superoxide dismutase (SOD) in the cell were measured by spectroscopy, respectively. The degress of DNA damage was evaluated with single cell gel electrophoresis (SCGE) technique.
Total flavonoids of Hedysarum polybortry could scavenge oxygen radicals. After being exposed to 100 micromol/L H2O2 for 4 hours, the level of LDH and MDA was increased significantly while SOD level was decreased. However, total flavonoids of Hedysarum polybotry could decrease the content of LDH, MDA and increase SOD activity significantly and inhibit the damage of DNA.
Total flavonoids of Hedysarum polybotry has protective effect on human umbilical vein endothelial cells injury induced by hydrogen peroxide,which may be related to increasing the antioxidant capacity of human umbilical vein endothelial cells.
探讨多序岩黄芪总黄酮对过氧化氢诱导的人脐静脉内皮细胞损伤的保护作用。
采用化学发光法检测多序岩黄芪总黄酮清除氧自由基的能力。分别用分光光度法测定培养基中乳酸脱氢酶（LDH）、细胞中丙二醛（MDA）和超氧化物歧化酶（SOD）的含量。用单细胞凝胶电泳（SCGE）技术评估DNA损伤程度。
多序岩黄芪总黄酮能清除氧自由基。细胞在100微摩尔/升过氧化氢中暴露4小时后，LDH和MDA水平显著升高，而SOD水平降低。然而，多序岩黄芪总黄酮能显著降低LDH、MDA含量，提高SOD活性，并抑制DNA损伤。
多序岩黄芪总黄酮对过氧化氢诱导的人脐静脉内皮细胞损伤具有保护作用，其机制可能与人脐静脉内皮细胞抗氧化能力增强有关。
