鸦胆子油对A549细胞系血管内皮生长因子表达的影响
[Effects of brucea javanica oil on expression of vascular endothelial growth factor in A549 cell line].
作者信息
Xu Xiang, Xu Dong-Hang, Jiang Bo, Lv Qing-Hua
机构信息
Division of Clinical Pharmacy, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Nov;33(21):2517-20.
OBJECTIVE
To study the effects of brucea javanica oil on the expression of vascular endothelial growth factor (VEGF) in A549 cell line.
METHOD
A549 cells were incubated with different concentrations of brucea javanica oil (0.5, 1.25, 2.5, 5 g x L(-1) for 48 h respectively. VEGF level in supernatant was determined by VEGF ELISA kits and mRNA expression of VEGF was evaluated by RT-PCR. PMN in health volunteers was treated as control groups.
RESULT
Supernatant VEGF protein and mRNA expression were significantly elevated in A549 cells compared with the mononuclear cells (120.73 vs 21.21, P < 0.05). Brucea javanica oil (2.5 g x L(-1)) could reduced supernatant VEGF protein in A549 cells (20.30 vs 120.73, P < 0.05), but had no effect on the expression of VEGF mRNA (1.0230 vs 0.9573). It was found that brucea javanica oil (5 g x L(-1)) significantly reduced VEGF mRNA expression (0.4682 vs 0.9573, P < 0.05).
CONCLUSION
Brucea javanica oil can depress the VEGF mRNA expression and secretion in A549 cells, which may be one of the mechanisms of its antitumor effect.
目的
研究鸦胆子油对A549细胞系中血管内皮生长因子（VEGF）表达的影响。
方法
将A549细胞分别用不同浓度的鸦胆子油（0.5、1.25、2.5、5 g·L⁻¹）孵育48小时。用VEGF ELISA试剂盒测定上清液中VEGF水平，并用RT-PCR评估VEGF的mRNA表达。将健康志愿者的中性粒细胞作为对照组。
结果
与单核细胞相比，A549细胞中的上清液VEGF蛋白和mRNA表达显著升高（120.73对21.21，P < 0.05）。鸦胆子油（2.5 g·L⁻¹）可降低A549细胞中的上清液VEGF蛋白（20.30对120.73，P < 0.05），但对VEGF mRNA的表达无影响（1.0230对0.9573）。发现鸦胆子油（5 g·L⁻¹）显著降低VEGF mRNA表达（0.4682对0.9573，P < 0.05）。
结论
鸦胆子油可抑制A549细胞中VEGF mRNA的表达和分泌，这可能是其抗肿瘤作用的机制之一。
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