基于电荷耦合器件的光纤光谱仪检测

CCD based fiber-optic spectrometer detection.

作者信息

Kapoor Rakesh

机构信息

Department of Physics, University of Alabama at Birmingham, Birmingham, AL, USA.

出版信息

Methods Mol Biol. 2009;503:435-45. doi: 10.1007/978-1-60327-567-5_25.

DOI:10.1007/978-1-60327-567-5_25

PMID:19151957

Abstract

Highly sensitive and cost effective measurement tools are required in biotechnology research and applications. Fluorescence provides very simple, cost effective, and sensitive methods in most of the biosensor techniques. Spectrometer is an essential tool for any kind of spectroscopic measurements. A charged coupled device (CCD)-based fiber optic spectrometer is highly compact, light weight, and an extremely easy to use tool. In this chapter, we have described the use of CCD-based fiber-optic spectrometers in detection of fluorescence signal from a fiber-optic-based sensor. The method can easily be extended to fluorescence detection in any other application.

摘要

生物技术研究和应用需要高灵敏度且经济高效的测量工具。在大多数生物传感器技术中，荧光提供了非常简单、经济高效且灵敏的方法。光谱仪是任何光谱测量的必备工具。基于电荷耦合器件（CCD）的光纤光谱仪高度紧凑、重量轻，且使用极其简便。在本章中，我们描述了基于CCD的光纤光谱仪在检测基于光纤的传感器的荧光信号中的应用。该方法可轻松扩展到任何其他应用中的荧光检测。