Chandrasekaran A, Packirisamy M
Optical Microsystems Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montréal, Canada.
IEE Proc Nanobiotechnol. 2006 Dec;153(6):137-43. doi: 10.1049/ip-nbt:20060012.
The advantages of integrating microfluidics into photonics-based biosensing for fabricating microreactor type lab-on-a-chip devices carries a lot of advantages, such as smaller sample volume handling, controlled drug delivery and high throughput diagnosis, which is useful for in situ medical diagnosis and point-of-care (POC) testing. A hybrid integrated optical microfluidic system has been developed for the study of single molecules and enzymatic reactions. The method of optical absorption has been employed for biosensing and the feasibility of absorption-based detection on the microfluidic platform has been demonstrated using horseradish peroxidase and hydrogen peroxide, as an example. The results show that the device is useful for the analysis of both the individual chemical specimen and also the study of chemical and biological reaction between two reacting species. The hybrid integration of microfluidics and optical ensembles thus forms the basis for developing the microreactor type lab-on-a-chip device, which would have several important applications in the area of nanobiotechnology.
将微流控技术集成到基于光子学的生物传感中以制造微反应器型芯片实验室设备具有诸多优势，例如能够处理更小体积的样本、实现可控的药物递送以及进行高通量诊断，这对于原位医学诊断和即时检测（POC）非常有用。一种用于研究单分子和酶促反应的混合集成光学微流控系统已经被开发出来。光吸收方法已被用于生物传感，并且以辣根过氧化物酶和过氧化氢为例，证明了在微流控平台上基于吸收检测的可行性。结果表明，该设备对于单个化学样本的分析以及两种反应物之间的化学和生物反应研究都很有用。因此，微流控技术与光学组件的混合集成构成了开发微反应器型芯片实验室设备的基础，该设备在纳米生物技术领域将有若干重要应用。
