Arici D S, Tuncer E, Ozer H, Simek G, Koyuncu A
Cumhuriyet University School of Medicine, Department of Pathology, Sivas, Turkey.
Neoplasma. 2009;56(1):63-7. doi: 10.4149/neo_2009_01_63.
Abnormal regulation of the cell cycle is a feature of many neoplasms. The role of cell cycle regulators in oncogenesis has been investigated in many human tumors. Alteration of the retinoblastoma (pRb) and cyclin D1 disrupt the Rb pathway and occur in many carcinomas. However the expression of the Rb and cyclin D1 in intestinal type gastric carcinoma is unclear. The purpose of this study was to investigate the expression of Rb and cyclinD1 in resected gastric carcinoma, their adjacent nonneoplastic mucosa and normal gastric mucosa, and finally to provide insights into the role of the Rb and cyclin D1 in gastric carcinogenesis. We investigated Rb and cyclin D1 expression in 43 patients (32 men, 11 women; mean age: 64) with primary gastric adenocarcinoma and compared the results with adjacent nonneoplastic mucosa. Adjacent nonneoplastic mucosa consisted of atrophy, dysplasia, intestinal metaplasia and gastritis. Expression of Rb was detected in 30 (69.7%) of gastric carcinoma, 18 (41.8%) of the adjacent nonneoplastic mucosa. Expression of cyclinD1 protein was detected in 31 (72%) of gastric carcinoma, 24 (55.8%) of adjacent nonneoplastic mucosa. Expression of Rb and cyclinD1 was not detected in normal gastric mucosa. The positive rate of Rb and cyclin D1 expression in gastric carcinoma was significantly higher than that adjacent nonneoplastic mucosa (p<0.05). There were significant trends for increased expression of Rb and cyclinD1 from nonneoplastic mucosa including atrophy, dyplasia, intestinal metaplasia and gastritis to carcinoma. These results suggested that positive expression of pRb and cyclinD1 might be an early event in gastric carcinoma and it tend to begin at precursor lesions and maintain throughout the progression of infiltration. Key words: Retinoblastoma, cyclin D1, gastric carcinoma, dysplasia, atrophy, intestinal metaplasia.
细胞周期的异常调控是许多肿瘤的一个特征。细胞周期调节因子在肿瘤发生中的作用已在许多人类肿瘤中得到研究。视网膜母细胞瘤（pRb）和细胞周期蛋白D1的改变会破坏Rb通路，且在许多癌症中都会出现。然而，Rb和细胞周期蛋白D1在肠型胃癌中的表达尚不清楚。本研究的目的是调查Rb和细胞周期蛋白D1在切除的胃癌、其相邻的非肿瘤性黏膜和正常胃黏膜中的表达情况，最终深入了解Rb和细胞周期蛋白D1在胃癌发生中的作用。我们调查了43例（32例男性，11例女性；平均年龄：64岁）原发性胃腺癌患者中Rb和细胞周期蛋白D1的表达情况，并将结果与相邻的非肿瘤性黏膜进行比较。相邻的非肿瘤性黏膜包括萎缩、发育异常、肠化生和胃炎。在30例（69.7%）胃癌、18例（41.8%）相邻的非肿瘤性黏膜中检测到Rb的表达。在31例（72%）胃癌、24例（55.8%）相邻的非肿瘤性黏膜中检测到细胞周期蛋白D1蛋白的表达。在正常胃黏膜中未检测到Rb和细胞周期蛋白D1的表达。胃癌中Rb和细胞周期蛋白D1表达的阳性率显著高于相邻的非肿瘤性黏膜（p<0.05）。从包括萎缩、发育异常、肠化生和胃炎在内的非肿瘤性黏膜到癌，Rb和细胞周期蛋白D1的表达有显著增加的趋势。这些结果表明，pRb和细胞周期蛋白D1的阳性表达可能是胃癌中的一个早期事件，且它倾向于在前体病变时开始并在浸润进展过程中持续存在。关键词：视网膜母细胞瘤；细胞周期蛋白D1；胃癌；发育异常；萎缩；肠化生
