Kohl S, Starr S E, oleske J M, Shore S L, Ashman R B, Nahmias A J
J Immunol. 1977 Mar;118(3):729-35.
Human peripheral blood mononuclear cells which mediate antibody-dependent cellular cytotoxicity (ADCC) against herpes simplex virus- (HSV) infected target cells consist of both adherent (MA) and nonadherent (MNA) effector cell populations. These two cell populations can be distinguished by their different phagocytic properties and morphologic appearance, their requirement for antibody in the ADCC reaction, and the rapidity with which they lyse target cells in the presence of immune serum. The MA cells are predominantly phagocytic and have the morphologic characteristics of monocyte-macrophages, whereas the MNA cells are nonphagocytic and appear to be small to medium-sized lymphocytes. Optimal expression of ADCC by MA cells requires higher concentrations of immune serum than does MNA cell-mediated ADCC. MA-mediated cell killing is first detectable by 8 hr and reaches completion after 24 hr of incubation. In contrast, MNA-mediated ADCC produces target cell damage by 2 hr and reaches completion at 8 hr of incubation. Unlike MNA effector cells, the MA effector cells are profoundly inhibited after preincubation with either latex or silica particles. The HSV immune status of the donor had no effect on the ability of either cell population to mediate ADCC. These data demonstrate the participation of both nonadherent mononuclear cells, presumably K cells, and monocyte-macrophages, in ADCC directed against HSV-infected target cells.
介导针对单纯疱疹病毒（HSV）感染靶细胞的抗体依赖性细胞毒性（ADCC）的人外周血单核细胞由贴壁效应细胞群体（MA）和非贴壁效应细胞群体（MNA）组成。这两个细胞群体可通过其不同的吞噬特性和形态外观、ADCC反应中对抗体的需求以及在免疫血清存在下裂解靶细胞的速度来区分。MA细胞主要具有吞噬作用，具有单核细胞 - 巨噬细胞的形态特征，而MNA细胞不具有吞噬作用，似乎是小到中等大小的淋巴细胞。与MNA细胞介导的ADCC相比，MA细胞实现ADCC的最佳表达需要更高浓度的免疫血清。MA介导的细胞杀伤在孵育8小时后首次可检测到，并在孵育24小时后完成。相比之下，MNA介导的ADCC在孵育2小时时就会对靶细胞造成损伤，并在孵育8小时时完成。与MNA效应细胞不同，MA效应细胞在用乳胶或二氧化硅颗粒预孵育后会受到显著抑制。供体的HSV免疫状态对这两种细胞群体介导ADCC的能力均无影响。这些数据表明，非贴壁单核细胞（可能是K细胞）和单核细胞 - 巨噬细胞均参与了针对HSV感染靶细胞的ADCC。
