Catalona W J, Ratliff T L, McCool R E
Immunology. 1980 Jan;39(1):119-25.
We examined the nature of effector cells that mediate antibody-dependent cell-mediated cytotoxicity (ADCC) and spontaneous cell-mediated cytotoxicity (SCMC) against the 253J human bladder transitional cell carcinoma cell line. Fractionation of peripheral blood lymphocytes (PBL) showed that ADCC and SCMC against the 253J targets were mediated by non-adherent, surface immunoglobulin-negative lymphocytes lacking or having only weak affinity for sheep erythrocytes. Macrophages were not cytotoxic against 253J cells in 18 h culture, as removal of macrophages by glass adherence or treatment with silica particles did not reduce ADCC or SCMC, and macrophage-enriched effector cell preparations were only weakly cytotoxic.
我们研究了介导针对253J人膀胱移行细胞癌细胞系的抗体依赖性细胞介导的细胞毒性（ADCC）和自发细胞介导的细胞毒性（SCMC）的效应细胞的性质。外周血淋巴细胞（PBL）分级分离显示，针对253J靶标的ADCC和SCMC由对绵羊红细胞缺乏亲和力或仅有弱亲和力的非黏附性、表面免疫球蛋白阴性淋巴细胞介导。在18小时培养中，巨噬细胞对253J细胞无细胞毒性，因为通过玻璃黏附去除巨噬细胞或用二氧化硅颗粒处理并不会降低ADCC或SCMC，且富含巨噬细胞的效应细胞制剂仅有微弱的细胞毒性。
