Ravindra P V, Tiwari Ashok K, Ratta Barkha, Chaturvedi Uttara, Palia Sudesh Kumar, Chauhan R S
Molecular Biology Laboratory, Division of Animal Biotechnology, Indian Veterinary Research Institute, Izatnagar, 243122 UP, India.
Virus Res. 2009 Apr;141(1):13-20. doi: 10.1016/j.virusres.2008.12.008. Epub 2009 Jan 17.
The velogenic Newcastle disease virus (NDV) causes highly infectious and economically significant Newcastle disease (ND) in birds of various species. In cell culture NDV induces cytopathic effect (CPE) characterized by rounding, vacuolation, syncytia formation and cell death. Aside from cell to cell fusion caused by the F and HN glycoprotein of the virus molecular events leading to cell death are not known. In the current study, NDV-infected Vero cells, at 48 h p.i., showed nuclear condensation, cytoplasm blebbing, DNA fragmentation, and phosphatidylserine translocation to the cell surface. In addition, virus-infected cells demonstrated decreased DNA content and an increased Bax to Bcl-2 ratio, p53 level and caspase 3, 8, 9 expression compared to mock-infected cells. Based on these results, it was concluded that CPE in NDV-infected cells was caused by to the induction of apoptosis with the involvement of p53 and the Bax, dependent apoptotic pathways.
速发型新城疫病毒（NDV）可在多种禽类中引发高传染性且具有重大经济影响的新城疫（ND）。在细胞培养中，NDV可诱导细胞病变效应（CPE），其特征为细胞变圆、空泡化、形成多核巨细胞及细胞死亡。除了由病毒的F和HN糖蛋白引起的细胞间融合外，导致细胞死亡的分子事件尚不清楚。在本研究中，感染NDV的Vero细胞在感染后48小时显示出核浓缩、细胞质起泡、DNA片段化以及磷脂酰丝氨酸向细胞表面的易位。此外，与 mock 感染的细胞相比，病毒感染的细胞显示出DNA含量降低，Bax与Bcl-2的比率、p53水平以及半胱天冬酶3、8、9的表达增加。基于这些结果，得出结论：NDV感染细胞中的CPE是由p53和Bax依赖性凋亡途径诱导的细胞凋亡所致。
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