Hill Sharon R, Hansson Bill S, Ignell Rickard
Division of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, Box 102, SE-23053 Alnarp, Sweden.
Chem Senses. 2009 Mar;34(3):231-52. doi: 10.1093/chemse/bjn080. Epub 2009 Jan 19.
Culex quinquefasciatus, the southern house mosquito, is highly dependent on its olfactory system for vector-related activities such as host seeking and oviposition. The antennae are the primary olfactory organs in mosquitoes. We describe 5 morphological types of sensilla on the antenna of C. quinquefasciatus: 1) a pair of sensilla coeloconica located at the distal tip, 2) long and short sensilla chaetica present on all 13 antennal flagella, 3) sensilla ampullacea found on the 2 proximal-most flagella, 4) 2 morphological types of grooved pegs dispersed throughout the flagella, and 5) 5 morphological subtypes of sensilla trichodea distributed among all flagella. Antennal trichoid and grooved peg sensilla of mosquitoes have been demonstrated to house the olfactory receptor neurons (ORNs) that detect many of the odors involved in eliciting vector-related behaviors. In order to initiate the functional characterization of the peripheral olfactory system in female C. quinquefasciatus, we mapped the physiological responses of all 5 morphological subtypes of sensilla trichodea to an odor panel of 44 behaviorally relevant odor compounds. We identified 17 functional classes of sensilla trichodea: 3 short sharp-tipped, 9 short blunt-tipped type I, and 5 short blunt-tipped type II sensilla. One morphological subtype remains unclassified as the long sharp-tipped sensilla did not respond to any of the volatiles tested. The functional classes of the ORNs were analyzed with respect to stimulus response profiles, stimuli sensitivity, and temporal coding patterns. Comparisons with other functionally classified mosquito antennal sensilla trichodea are discussed.
致倦库蚊，即南方家蚊，在诸如寻找宿主和产卵等与媒介相关的活动中高度依赖其嗅觉系统。触角是蚊子的主要嗅觉器官。我们描述了致倦库蚊触角上5种形态类型的感器：1）位于远端尖端的一对腔锥感器；2）存在于所有13节触角鞭节上的长和短的毛形感器；3）在最靠近基部的2节鞭节上发现的壶形感器；4）遍布鞭节分布的2种形态类型的槽形栓锥感器；5）分布于所有鞭节上的5种形态亚型的毛形感器。已证明蚊子的触角毛形和槽形栓锥感器容纳了检测许多引发与媒介相关行为的气味的嗅觉受体神经元（ORN）。为了启动对雌性致倦库蚊外周嗅觉系统的功能表征，我们将所有5种形态亚型的毛形感器对44种与行为相关的气味化合物气味组的生理反应进行了图谱绘制。我们确定了毛形感器的17种功能类别：3种短尖型、9种短钝尖I型和5种短钝尖II型感器。一种形态亚型仍未分类，因为长尖型感器对所测试的任何挥发物都没有反应。针对刺激反应图谱、刺激敏感性和时间编码模式对ORN的功能类别进行了分析。讨论了与其他功能分类的蚊子触角毛形感器的比较。
