Wang Ming, Gu Xinggui, Zhang Guanxin, Zhang Deqing, Zhu Daoben
Beijing National Laboratory for Molecular Sciences, Organic Solids Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Langmuir. 2009 Feb 17;25(4):2504-7. doi: 10.1021/la803870v.
We report herein a new colorimetric assay method for acetylcholinesterase (AChE) activity and its inhibitor screening by making use of the following facts: (1) the aggregation of gold nanoparticles (Au-NPs) results in the red-shift of the plasmon absorption due to interparticle plasmon interactions and (2) AChE can catalyze the hydrolysis of acetylthiocholine into thiocholine which can induce the aggregation of Au-NPs. With this convenient method, the activity of AChE with a concentration as low as 0.6 mU/mL can be assayed. Moreover, this assay method is also useful for screening inhibitors of AChE. Given its simplicity and easy-operation, this method may extend to high-throughput screening of AChE inhibitors and relevant drug discovery.
我们在此报告一种用于乙酰胆碱酯酶（AChE）活性及其抑制剂筛选的新型比色测定方法，该方法利用了以下事实：（1）金纳米颗粒（Au-NPs）的聚集由于颗粒间等离子体相互作用导致等离子体吸收发生红移；（2）AChE可催化乙酰硫代胆碱水解为硫代胆碱，后者可诱导Au-NPs聚集。通过这种简便方法，可测定低至0.6 mU/mL浓度的AChE活性。此外，该测定方法也可用于筛选AChE抑制剂。鉴于其简单性和易操作性，该方法可能扩展到AChE抑制剂的高通量筛选及相关药物发现。
