Department of Physical Chemistry, Israel Institute for Biological Research, P.O. Box 19, Ness-Ziona 74100, Israel.
Anal Chim Acta. 2011 Oct 10;703(2):234-8. doi: 10.1016/j.aca.2011.07.033. Epub 2011 Jul 28.
A new sensitive surface-enhanced Raman scattering (SERS) assay for detection of cholinesterase inhibitors such as organophosphorous pesticides using silver colloidal nanoparticles was developed and optimized. Acetylcholinesterase (AChE) mediated the hydrolysis of acetylthiocholine to produce thiocholine, which interacted with the silver nanoparticles to give a specific SERS spectrum. Variation in enzyme activity due to inhibition was measured from changes in intensity of a characteristic peak (772 cm(-1)) of the SERS spectrum that was directly correlated with the concentration of produced thiocholine. The method was demonstrated for the detection of paraoxon as reference AChE inhibitor. Limit of detection of paraoxon for 5 min incubation at 25 °C was 1.8×10(-8) M. This assay can be utilized for the detection of trace amounts of any AChE inhibitor.
一种新的灵敏表面增强拉曼散射（SERS）分析方法被开发和优化，用于检测诸如有机磷农药等胆碱酯酶抑制剂。利用银胶体纳米粒子，乙酰胆碱酯酶（AChE）介导将乙酰硫代胆碱水解产生硫代胆碱，硫代胆碱与银纳米粒子相互作用产生特定的 SERS 光谱。由于抑制作用导致的酶活性变化通过 SERS 光谱中特征峰（772 cm(-1))的强度变化来测量，该特征峰的强度与产生的硫代胆碱的浓度直接相关。该方法用于检测对氧磷作为参考 AChE 抑制剂。在 25°C 孵育 5 分钟时，对氧磷的检测限为 1.8×10(-8) M。该检测方法可用于检测任何痕量的 AChE 抑制剂。
