Zeugolis D I, Paul R G, Attenburrow G
Tissue Modulation Laboratory, National University of Singapore Tissue Engineering Programme, National University of Singapore, 117510 Singapore.
J Biomater Sci Polym Ed. 2009;20(2):219-34. doi: 10.1163/156856209X404505.
Extruded collagen fibres have been shown to be a competitive biomaterial for tissue-engineering applications. Since different tissues are coming in different textures, as far as it is concerned their fibre diameter and consequently their mechanical properties, herein we aim to investigate the influence of the collagen concentration and the amount of NaCl on the properties of these fibres. Scanning electron microscopy study revealed that the substructure of the collagen fibres was the same, regardless of the treatment. The thermal properties were found to be independent of the collagen concentration or the amount of NaCl utilized (P > 0.05). An inversely proportional relationship between dry fibre diameter and stress at break was observed. Increasing the collagen concentration yielded fibres with significant higher diameter (P < 0.002), strain (P < 0.009) and force (P < 0.001) values, whilst the stress (P < 0.008) and modulus (P < 0.009) values were decreased. For the fabrication of fibres with reproducible properties, 20% NaCl was found to be the optimum. Overall, reconstituted collagen fibres were produced with properties similar to native or synthetic fibres to suit a wide range of tissue-engineering applications.
已证明挤出的胶原纤维是一种适用于组织工程应用的具有竞争力的生物材料。由于不同组织具有不同质地，就其纤维直径以及由此产生的机械性能而言，在此我们旨在研究胶原浓度和氯化钠用量对这些纤维性能的影响。扫描电子显微镜研究表明，无论处理方式如何，胶原纤维的亚结构都是相同的。发现热性能与所使用的胶原浓度或氯化钠用量无关（P>0.05）。观察到干纤维直径与断裂应力之间呈反比关系。增加胶原浓度会使纤维的直径（P<0.002）、应变（P<0.009）和力（P<0.001）值显著更高，而应力（P<0.008）和模量（P<0.009）值则降低。对于制备具有可重复性能的纤维，发现20%的氯化钠是最佳用量。总体而言，制备出了具有与天然或合成纤维相似性能的再生胶原纤维，以适用于广泛的组织工程应用。
