Zeugolis D I, Paul R G, Attenburrow G
Tissue Modulation Laboratory, National University of Singapore Tissue Engineering Programme, National University of Singapore, 117510, Singapore.
Acta Biomater. 2008 Nov;4(6):1646-56. doi: 10.1016/j.actbio.2008.05.015. Epub 2008 Jun 11.
Extruded collagen fibres have been shown to constitute a biomimetic three-dimensional scaffold with numerous tissue engineering applications. The multi-step fabrication process of this material provides opportunities for further advancements to improve the properties of the final product. Herein we investigated the influence of the post-self-assembly washing baths on the structural, mechanical and thermal properties of these fibres. The surface morphology and the inter-fibre packing were similar for every treatment. The overnight incubation in isopropanol yielded fibres with the highest temperature and energy of denaturation (p<0.013). Typical s- and j-shape stress-strain curves were obtained for all treatments in the dry and wet state respectively. Rehydration of the fibres resulted in increased fibre diameter (p<0.006) and reduced stress (p<0.001), force (p<0.001) and modulus (p<0.002) values for every treatment. In the dry state, the alcohol-treated fibres were characterized by the highest stress (p<0.002) values; whilst in the wet state the Tris-HCl-treated fibres were the weakest (p<0.006). For every treatment, in both dry and wet state, a strong and inverse relationship between the fibre diameter and the stress at break was observed. Overall, the fibres produced were characterized by properties similar to those of native tissues.
已证明挤出的胶原纤维构成了一种具有众多组织工程应用的仿生三维支架。这种材料的多步制造工艺为进一步改进以提高最终产品的性能提供了机会。在此，我们研究了自组装后洗涤浴对这些纤维的结构、力学和热性能的影响。每种处理的表面形态和纤维间堆积情况相似。在异丙醇中过夜孵育得到的纤维具有最高的变性温度和能量（p<0.013）。分别在干燥和湿润状态下，所有处理均获得了典型的s形和j形应力-应变曲线。纤维复水导致每种处理的纤维直径增加（p<0.006），应力（p<0.001）、力（p<0.001）和模量（p<0.002）值降低。在干燥状态下，经酒精处理的纤维具有最高的应力（p<0.002）值；而在湿润状态下，经Tris-HCl处理的纤维最弱（p<0.006）。对于每种处理，在干燥和湿润状态下，均观察到纤维直径与断裂应力之间存在强烈的反比关系。总体而言，所生产的纤维具有与天然组织相似的特性。
