秘鲁高流行地区用于人类嗜T淋巴细胞病毒感染常规诊断的三种酶联免疫吸附测定法的比较

Comparison of three ELISAs for the routine diagnosis of human T-lymphotropic virus infection in a high-prevalence setting in Peru.

作者信息

Verdonck Kristien, González Elsa, Maldonado Fernando, Agapito Doris, Van Dooren Sonia, Vandamme Anne-Mieke, Silva-Santisteban Alfonso, Vanham Guido, Clark Daniel, Gotuzzo Eduardo

机构信息

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Av. Honorio Delgado 430, Lima 31, Peru.

出版信息

Trans R Soc Trop Med Hyg. 2009 Apr;103(4):420-2. doi: 10.1016/j.trstmh.2008.12.002. Epub 2009 Jan 19.

DOI:10.1016/j.trstmh.2008.12.002

PMID:19155035

Abstract

To compare three human T-lymphotropic virus (HTLV) ELISAs in a high-prevalence setting, we recruited 300 adults: 125 relatives of HTLV-infected subjects and 175 patients with possible diagnoses of HTLV-associated diseases. Sera were tested with Platelia, Murex, and Ortho ELISA. Samples with positive or discordant ELISA underwent confirmatory Inno-Lia testing. Inno-Lia gave 85/300 HTLV-1-positive and 1/300 HTLV-2-positive results. The positive predictive value was 98% for Platelia, and 100% for Murex and Ortho. Six samples had discordant ELISA; Murex gave one false-negative result, Ortho two and Platelia one. In high-prevalence settings, it is recommended to test samples with two ELISAs before considering them HTLV-seronegative.

摘要

为了在高流行率环境中比较三种人类嗜T淋巴细胞病毒（HTLV）酶联免疫吸附测定（ELISA）方法，我们招募了300名成年人：125名HTLV感染受试者的亲属和175名可能被诊断为HTLV相关疾病的患者。血清分别用Platelia、Murex和Ortho ELISA进行检测。ELISA检测结果为阳性或不一致的样本接受了Inno-Lia确证检测。Inno-Lia检测出85/300例HTLV-1阳性和1/300例HTLV-2阳性结果。Platelia的阳性预测值为98%，Murex和Ortho的阳性预测值为100%。有6个样本的ELISA检测结果不一致；Murex出现1例假阴性结果，Ortho出现2例假阴性结果，Platelia出现1例假阴性结果。在高流行率环境中，建议在将样本视为HTLV血清阴性之前先用两种ELISA方法进行检测。