Rovensky Y A, Bershadsky A D, Givargizov E I, Obolenskaya L N, Vasiliev J M
Cancer Research Center, Academy of Medical Sciences of the USSR, Moscow.
Exp Cell Res. 1991 Nov;197(1):107-12. doi: 10.1016/0014-4827(91)90486-e.
Mouse embryo fibroblasts were cultivated on special substrates with discontinuous surfaces. The substrates were silicon plates with multiple vertical (65-90 microns height) spike-like silicon microcrystals evenly distributed on the plate surfaces. It was shown that the cells were successfully spread and flattened on these substrates. The spread cells formed several discrete attachment zones at the tops and side surfaces of the spikes; these zones were separated from one another by distances considerably greater than the diameter of the unspread cell. At early stages of spreading the unspread cells attached to the tops of single spikes and extended long filopodia attached to the distant spikes. At later stages the lamellae were formed between the filopodia: probably these filopodia served as guidelines for extension of lamellae and progressive cell spreading. These experiments demonstrated that continuity of substrate surface is not a necessary condition for advanced cell spreading.
小鼠胚胎成纤维细胞在具有不连续表面的特殊基质上培养。这些基质是硅板，板表面均匀分布着多个垂直（高度为65 - 90微米）的尖状硅微晶。结果表明，细胞在这些基质上成功铺展并变平。铺展的细胞在尖状物的顶部和侧面形成了几个离散的附着区域；这些区域彼此之间的距离远大于未铺展细胞的直径。在铺展的早期阶段，未铺展的细胞附着在单个尖状物的顶部，并伸出长丝状伪足附着在远处的尖状物上。在后期阶段，丝状伪足之间形成了片状伪足：可能这些丝状伪足为片状伪足的延伸和细胞的逐步铺展提供了引导。这些实验表明，基质表面的连续性不是细胞高级铺展的必要条件。
