Su Wen-Ta, Chu I-Ming, Yang Jung-Yen, Lin Chuan-Ding
Department of Chemical Engineering, National Taipei University of Technology, No. 1, Sec. 3, Chung-Hsiao E. Rd., Taipei, Taiwan 106, China.
Micron. 2006;37(8):699-706. doi: 10.1016/j.micron.2006.03.007. Epub 2006 Apr 3.
Fibroblasts alter their shape, direction of movement, cytoskeleton arrangement, and focal contact when placed upon square array pillars. We prepared pillars of 1 microm diameter, separated by 3 microm, and having 1, 5, and 10 microm heights using substrates displaying identical surface chemistry. When cells seeded initially onto the tops of the pillars, fibroblasts subsequently were immobilized in situ by several pillars that visibly protruded through, but did not pierce, the cell bodies. The cytoplasma then migrated outward with long straight lamella along the interval of the pillars and formed several discrete attachment zones at their side walls - the value of their form index (FI) was as high as 35 - which altered the cellular shape entirely. Most of the cells interacted with the pillar substrate by spreading preferentially in a particular direction, but some of them had the ability to undergo coincident two-direction (x and y) migration; right-angle turn orientations led to the growth of dramatic cellular morphologies. Interestingly, this fibroblast's behavior variation was gradually in proportion to the pillar height of substrate. Our results confirm that cellular migration and cellular shape are both strongly affected by the geometry of the growth microenvironment.
当置于方形阵列柱上时，成纤维细胞会改变其形状、运动方向、细胞骨架排列和粘着斑。我们使用具有相同表面化学性质的基质制备了直径为1微米、间距为3微米、高度分别为1微米、5微米和10微米的柱子。当细胞最初接种到柱子顶部时，成纤维细胞随后被几根明显穿过但未刺穿细胞体的柱子原位固定。然后细胞质沿着柱子的间隔向外迁移，形成长而直的薄片，并在其侧壁形成几个离散的附着区域——其形态指数（FI）高达35——这完全改变了细胞形状。大多数细胞通过优先沿特定方向铺展与柱形基质相互作用，但其中一些细胞具有同时进行双向（x和y）迁移的能力；直角转向导致形成显著的细胞形态。有趣的是，这种成纤维细胞的行为变化与基质的柱高成比例逐渐变化。我们的结果证实，细胞迁移和细胞形状都受到生长微环境几何形状的强烈影响。
