包含天然核糖开关适体结构域的人工核酶开关
Artificial ribozyme switches containing natural riboswitch aptamer domains.
作者信息
Wieland Markus, Benz Armin, Klauser Benedikt, Hartig Jörg S
机构信息
Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Universitätsstrasse 10, 78457 Konstanz, Germany.
出版信息
Angew Chem Int Ed Engl. 2009;48(15):2715-8. doi: 10.1002/anie.200805311.
RNA Lego: The use of natural riboswitch aptamers in synthetic RNA switches (see picture) should broaden the scope of artificial RNA regulators dramatically. It is shown that thiamine pyrophosphate (TPP) aptamers can be used in engineered devices as very sensitive switches of gene expression in unmodified organisms. The approach demonstrates that intrinsic metabolites can be utilized as external effectors of cellular functions.
RNA乐高积木：天然核糖开关适体在合成RNA开关中的应用（见图）将极大地拓宽人工RNA调控因子的范围。研究表明，硫胺素焦磷酸（TPP）适体可用于工程化装置，作为未修饰生物体中基因表达的非常灵敏的开关。该方法表明，内源性代谢物可被用作细胞功能的外部效应物。
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