基于中心复合设计和响应面法的荷叶中荷叶碱与黄酮的最佳提取工艺
[Optimal extraction of nuciferine and flavone from lotus leaf based on central composite design and response surface methodology].
作者信息
Wang Guo-Hua, Zhang Bao-Xian, Nie Qi-Xia, Li Hui, Zang Chen
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Oct;33(20):2332-5.
OBJECTIVE
To optimize the process of extracting effective constituents from lotus leaf.
METHOD
Independent variables were ethanol concentration reflux time and solvent fold, dependent variables were extraction rates of nuciferine and flavone in lotus leaf, central composite design and response surface methodology were used for optimization of extraction of lotus leaf.
RESULT
The optimum conditions of extraction process were 75% -80% ethanol, 2-3 hours for reflux, 20-25 fold solvent and 2 times for extraction. Bias between observed and predicted of rates of nuciferine and flavone values were 5.53%, -6.02%, respectively.
CONCLUSION
The values observed and predicted were close to each other, which proved that the optimization of of extraction of lotus leaf by central composite design and response surface methodology was reasonable and successful.
目的
优化荷叶有效成分的提取工艺。
方法
自变量为乙醇浓度、回流时间和溶剂倍数，因变量为荷叶中荷叶碱和黄酮的提取率，采用中心复合设计和响应面法对荷叶提取工艺进行优化。
结果
提取工艺的最佳条件为乙醇浓度75% - 80%、回流2 - 3小时、溶剂倍数20 - 25倍、提取2次。荷叶碱和黄酮提取率的实测值与预测值之间的偏差分别为5.53%、-6.02%。
结论
实测值与预测值接近，证明采用中心复合设计和响应面法对荷叶提取工艺进行优化是合理且成功的。
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