School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang, China.
School of Pharmacy, Hangzhou Medical College, Hangzhou, Zhejiang, China.
Ultrason Sonochem. 2022 May;86:106026. doi: 10.1016/j.ultsonch.2022.106026. Epub 2022 May 6.
Nuciferine is an important drug candidate for the treatment of many diseases. However, there is no general method for its low - cost production. In this work, a feasible method for the production of nuciferine from lotus leaf, using ultrasonic-assisted extraction-solid phase extraction (UAE-SPE) as extraction and cleanup procedure, was developed. Petroleum ether and silica gel have been successfully used as extraction solvent and adsorbent to integrate UAE with SPE, respectively. Except for filtration, no treatment (e.g. concentration and redissolution, etc) was needed on UAE extract before SPE and the effluents obtained in the loading process of SPE could be used as UAE extraction solvent without purification. No obvious decline in the extraction efficiency of UAE and adsorption capacity of SPE was observed at least for 5 runs, which provides a feasible way for the continuous production of nuciferine in industry, i.e. Cyclic UAE-SPE. Moreover, SPE column could be conveniently regenerated and reused without significant decline in its adsorption capacity at least for 5 cycles, which can be used to reduce the cost of the whole system further. In comparison with other cleanup procedures, Cyclic UAE-SPE showed apparent advantages in energy conservation and emission reduction. LLE and crystallization were applied to separate nuciferine from other impurities further. Under optimum conditions, the total recovery rate of nuciferine with a purity over 90.0% from lotus leaf reached 50.1%. All in all, the developed method has advantages in convenient operation, low cost, and high efficiency, thus, is fitting for the production of high purity nuciferine.
荷叶中荷叶碱的超声辅助提取-固相萃取制备
荷叶碱是治疗多种疾病的重要候选药物。然而，目前还没有低成本生产它的通用方法。在这项工作中，开发了一种从荷叶中生产荷叶碱的可行方法，该方法采用超声辅助提取-固相萃取（UAE-SPE）作为提取和净化程序。石油醚和硅胶已成功用作提取溶剂和吸附剂，分别将 UAE 与 SPE 集成在一起。除了过滤之外，在 SPE 之前，不需要对 UAE 提取物进行任何处理（例如浓缩和重溶等），并且 SPE 加载过程中获得的流出物可以无需纯化即可用作 UAE 提取溶剂。至少进行了 5 次循环后，UAE 的提取效率和 SPE 的吸附能力均没有明显下降，这为工业上连续生产荷叶碱提供了可行的方法，即循环 UAE-SPE。此外，SPE 柱可以方便地再生和重复使用，其吸附能力至少在 5 个循环内没有明显下降，这可以进一步降低整个系统的成本。与其他净化程序相比，循环 UAE-SPE 在节能和减排方面具有明显优势。液液萃取（LLE）和结晶进一步用于分离荷叶碱和其他杂质。在最佳条件下，荷叶中荷叶碱的总回收率达到 50.1%，纯度超过 90.0%。总之，所开发的方法在操作方便、成本低和效率高方面具有优势，因此适合高纯度荷叶碱的生产。
