蜡样芽孢杆菌寡聚-1,6-葡萄糖苷酶的过量生产、纯化及结晶
Overproduction, purification and crystallization of Bacillus cereus oligo-1,6-glucosidase.
作者信息
Watanabe K, Kitamura K, Hata Y, Katsube Y, Suzuki Y
机构信息
Department of Agricultural Chemistry, Kyoto Prefectural University, Japan.
出版信息
FEBS Lett. 1991 Sep 23;290(1-2):221-3. doi: 10.1016/0014-5793(91)81264-9.
The gene coding for oligo-1,6-glucosidase from Bacillus cereus ATCC7064 has been overexpressed in Escherichia coli MV1184 cells under the control of the lac promoter in the genetically engineered plasmid pBCE4-2. Oligo-1,6-glucosidase was purified in large quantities and was crystallized at 25 degrees C by using a hanging drop vapor diffusion method with 53% saturated ammonium sulfate. The crystals have the shape of hexagonal bipyramids and belong to the space group P6(2) or P6(4) with lattice constants of a = b = 106.1 A, c = 120.0 A and gamma = 120 degrees.
蜡样芽孢杆菌ATCC7064的寡聚-1,6-葡糖苷酶编码基因已在基因工程质粒pBCE4-2的乳糖启动子控制下在大肠杆菌MV1184细胞中过表达。大量纯化了寡聚-1,6-葡糖苷酶，并通过使用53%饱和硫酸铵的悬滴气相扩散法在25℃下使其结晶。晶体呈六方双锥体形状，属于空间群P6(2)或P6(4)，晶格常数为a = b = 106.1 Å，c = 120.0 Å，γ = 120°。
Zotero 插件