侧向流动分析的快速原型制作。

Rapid prototyping of lateral flow assays.

作者信息

Volkov Alexander, Mauk Michael, Corstjens Paul, Niedbala R Sam

机构信息

Department of Chemistry, Lehigh University, Bethlehem, PA, USA.

出版信息

Methods Mol Biol. 2009;504:217-35. doi: 10.1007/978-1-60327-569-9_14.

DOI:10.1007/978-1-60327-569-9_14

PMID:19159100

Abstract

Principles and characteristics of lateral flow strip assays are reviewed. Recent technology developments permit the use of inexpensive electronic readers for interrogating lateral flow strip test results, thus avoiding the inevitable variation and subjectivity of visual inspection to assess the capture of reporter-labeled analyte on test lines of the strip. Protocols for developing lateral flow assays are described, including two specific case studies for assaying cotinine (a small-molecule metabolite of nicotine) in a competitive format, and assaying HIV antibodies in a sandwich-type assay format.

摘要

综述了侧向流动试纸条检测的原理和特点。最近的技术发展使得可以使用价格低廉的电子阅读器来读取侧向流动试纸条的检测结果，从而避免了目视检查评估试纸条测试线上报告分子标记分析物捕获情况时不可避免的变异性和主观性。描述了开发侧向流动检测方法的方案，包括两个具体案例研究，一个是以竞争形式检测可替宁（尼古丁的小分子代谢物），另一个是以夹心型检测形式检测HIV抗体。