Zhou Yu, Pan Feng-Guang, Li Yan-Song, Zhang Yuan-Yuan, Zhang Jun-Hui, Lu Shi-Ying, Ren Hong-Lin, Liu Zeng-Shan
Key Laboratory of Zoonosis research, Ministry of Education, Institute of Zoonosis, Jilin University, Changchun 130062, PR China.
Biosens Bioelectron. 2009 Apr 15;24(8):2744-7. doi: 10.1016/j.bios.2009.01.034. Epub 2009 Feb 2.
One-step immunochromatographic assay using colloidal gold-labeled monoclonal antibody (Mab) probe for the rapid detection of brevetoxins (PbTxs) in fishery product samples was developed. The described assay was based on a competitive format using two antibodies. The primary antibody was conjugated with colloidal gold (detector reagent), the secondary antibody (capture reagent) was immobilized within a defined detection zone (control line) on a diagnostic cellulose nitrate membrane. The toxin in sample compete with immobilized toxin to bind with gold conjugated Mab. The mobile complex (colloidal gold-Mab-toxin) can be captured by the secondary antibody but cannot be captured by BSA-PbTx (test line). The color density of the test line correlated with the concentration of PbTx in sample in the range 10-4000 ng mL(-1). Spiked samples were detected by the assay and the visual detection limit was found to be 20 ng mL(-1). This qualitative test based on the visual evaluation of results did not require any equipment. The assay time for PbTx detection was less than 10 min, suitable for rapid testing on-site.
开发了一种使用胶体金标记单克隆抗体（Mab）探针的一步免疫层析测定法，用于快速检测水产品样品中的短裸甲藻毒素（PbTxs）。所述测定法基于使用两种抗体的竞争模式。一抗与胶体金偶联（检测试剂），二抗（捕获试剂）固定在诊断性硝酸纤维素膜上的特定检测区域（对照线）内。样品中的毒素与固定化毒素竞争结合金偶联的单克隆抗体。移动复合物（胶体金-Mab-毒素）可被二抗捕获，但不能被BSA-PbTx（测试线）捕获。测试线的颜色密度与样品中PbTx的浓度在10-4000 ng mL(-1)范围内相关。通过该测定法检测加标样品，目视检测限为20 ng mL(-1)。这种基于结果目视评估的定性测试不需要任何设备。检测PbTx的测定时间少于10分钟，适用于现场快速检测。
