Zhang Gaiping, Guo Junqing, Wang Xuannian
The Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, People's Republic of China.
Methods Mol Biol. 2009;504:169-83. doi: 10.1007/978-1-60327-569-9_12.
The immunochromatographic lateral flow strip test is a one-step test that facilitates low-cost, rapid identification of various analytes at the point of care. We have developed lateral flow strip tests for the specific qualitative or semiquantitative detection of antigens, antibodies, and haptens, such as drug residues. Here, we describe in detail the preparation of three examples of the strip tests for detection of (a) the infectious bursal disease virus; (b) Trichinella specific antibodies, and (c) Clenbuterol residues in urine samples.
免疫层析侧向流动试纸条检测是一种一步式检测方法,有助于在护理现场低成本、快速地鉴定各种分析物。我们已经开发出用于特异性定性或半定量检测抗原、抗体和半抗原(如药物残留)的侧向流动试纸条检测方法。在此,我们详细描述用于检测以下三种情况的试纸条检测方法的制备:(a) 传染性法氏囊病病毒;(b) 旋毛虫特异性抗体;(c) 尿液样本中的克伦特罗残留。