Wactawski-Wende Jean, Schisterman Enrique F, Hovey Kathleen M, Howards Penelope P, Browne Richard W, Hediger Mary, Liu Aiyi, Trevisan Maurizio
Department of Social and Preventive Medicine, School of Public Health and Health Professions, University at Buffalo, Buffalo, NY 14214, USA.
Paediatr Perinat Epidemiol. 2009 Mar;23(2):171-84. doi: 10.1111/j.1365-3016.2008.00985.x.
Studies in both human and animal species have suggested that oxidative stress may be associated with health outcomes, including the risk of infertility in both males and females. Sex hormones have been shown to have antioxidant properties. The difficulty in studying the role of oxidative stress in females is partly due to fluctuation in these endogenous sex hormones across the menstrual cycle. The aim of this study was to determine the association of oxidative stress levels with endogenous reproductive hormone levels and antioxidants, including vitamin levels, across the menstrual cycle in a prospective cohort of premenopausal women. The goal was to enroll 250 healthy, regularly menstruating premenopausal women for two menstrual cycles. Participants visited the clinic up to 8 times per cycle, at which time blood and urine were collected. The visits occurred at key hormonally defined phases of the menstrual cycle, with the help of an algorithm based on cycle length and data from a fertility monitor. In addition, participants were administered standardised questionnaires, had various physical measures taken, and had other pertinent data collected. A total of 259 women were enrolled in this study, with 250 completing two cycles, despite a demanding study protocol which participants were required to follow. This report describes the study design, baseline characteristics and visit completion rate for the BioCycle study.
对人类和动物物种的研究表明，氧化应激可能与健康结果相关，包括男性和女性的不孕风险。已证明性激素具有抗氧化特性。研究氧化应激在女性中的作用存在困难，部分原因是这些内源性性激素在月经周期中会发生波动。本研究的目的是在前瞻性队列研究中，确定绝经前女性在整个月经周期内氧化应激水平与内源性生殖激素水平以及抗氧化剂（包括维生素水平）之间的关联。目标是招募250名健康、月经规律的绝经前女性，进行两个月经周期的研究。参与者每个周期最多到诊所就诊8次，每次就诊时采集血液和尿液。在基于周期长度和生育监测器数据的算法帮助下，就诊时间安排在月经周期中关键的激素定义阶段。此外，还向参与者发放标准化问卷，进行各种身体测量，并收集其他相关数据。尽管研究方案要求苛刻，但共有259名女性参与了本研究，其中250名完成了两个周期的研究。本报告描述了BioCycle研究的研究设计、基线特征和就诊完成率。
