Kam Monica, Curtis Maurice A, McGlashan Susan R, Connor Bronwen, Nannmark Ulf, Faull Richard L M
Department of Anatomy with Radiology, Faculty of Medical and Health Sciences, The University of Auckland, Private Bag 92019, Auckland, New Zealand.
J Chem Neuroanat. 2009 May;37(3):196-205. doi: 10.1016/j.jchemneu.2008.12.009. Epub 2008 Dec 30.
The rostral migratory stream (RMS) is the major pathway by which progenitor cells migrate from the subventricular zone (SVZ) to the olfactory bulb (OB) in rodents, rabbits and primates. However, the existence of an RMS within the adult human brain has been elusive. Immunohistochemical studies utilising cell-type specific markers for early progenitor cells (CD133), proliferating cells (PCNA), astrocytes and type B cells (GFAP) and migrating neuroblasts (PSA-NCAM), reveal that the adult human RMS is organized into layers containing glial cells, proliferating cells and neuroblasts. In addition, the RMS is arranged around a remnant of the ventricular cavity that extends from the SVZ to the OB as seen by immunohistological staining analysis and electron microscopy, showing the presence of basal bodies and a typical 9+2 arrangement of tubulin in tufts of cilia from all levels of the RMS. Overall, these findings suggest that a pathway of migratory progenitor cells similar to that seen in other mammals is present within the adult human brain and that this pathway could provide for neurogenesis in the human forebrain. These findings contribute to the scientific understanding of adult neurogenesis and establish the detailed cytoarchitecture of this novel neurogenic niche in the human brain.
嘴侧迁移流（RMS）是啮齿动物、兔子和灵长类动物中祖细胞从脑室下区（SVZ）迁移至嗅球（OB）的主要途径。然而，成人大脑中RMS的存在一直难以捉摸。利用针对早期祖细胞（CD133）、增殖细胞（PCNA）、星形胶质细胞和B型细胞（GFAP）以及迁移的神经母细胞（PSA-NCAM）的细胞类型特异性标记物进行的免疫组织化学研究表明，成人的RMS被组织成包含神经胶质细胞、增殖细胞和神经母细胞的层。此外，通过免疫组织学染色分析和电子显微镜观察发现，RMS围绕着从SVZ延伸至OB的脑室腔残余部分排列，显示出RMS各水平的纤毛束中存在基体以及微管典型的9+2排列。总体而言，这些发现表明，成人大脑中存在一条类似于其他哺乳动物中所见的迁移祖细胞途径，并且该途径可能为人类前脑的神经发生提供条件。这些发现有助于对成体神经发生的科学理解，并确立了人脑中这个新型神经发生微环境的详细细胞结构。
