Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467-8601, Japan.
J Comp Neurol. 2011 Mar 1;519(4):690-713. doi: 10.1002/cne.22543.
The adult subventricular zone (SVZ) of the lateral ventricle contains neural stem cells. In rodents, these cells generate neuroblasts that migrate as chains toward the olfactory bulb along the rostral migratory stream (RMS). The neural-stem-cell niche at the ventricular wall is conserved in various animal species, including primates. However, it is unclear how the SVZ and RMS organization in nonhuman primates relates to that of rodents and humans. Here we studied the SVZ and RMS of the adult and neonatal common marmoset (Callithrix jacchus), a New World primate used widely in neuroscience, by electron microscopy, and immunohistochemical detection of cell-type-specific markers. The marmoset SVZ contained cells similar to type B, C, and A cells of the rodent SVZ in their marker expression and morphology. The adult marmoset SVZ had a three-layer organization, as in the human brain, with ependymal, hypocellular, and astrocyte-ribbon layers. However, the hypocellular layer was very thin or absent in the adult-anterior and neonatal SVZ. Anti-PSA-NCAM staining of the anterior SVZ in whole-mount ventricular wall preparations of adult marmosets revealed an extensive network of elongated cell aggregates similar to the neuroblast chains in rodents. Time-lapse recordings of marmoset SVZ explants cultured in Matrigel showed the neuroblasts migrating in chains, like rodent type A cells. These results suggest that some features of neurogenesis and neuronal migration in the SVZ are common to marmosets, humans, and rodents. This basic description of the adult and neonatal marmoset SVZ will be useful for future studies on adult neurogenesis in primates.
成年侧脑室室下区（SVZ）含有神经干细胞。在啮齿动物中，这些细胞产生神经母细胞，沿着嗅球的前向迁移流（RMS）呈链状迁移。脑室壁上的神经干细胞龛在包括灵长类动物在内的各种动物物种中都得到了保守。然而，非人类灵长类动物的 SVZ 和 RMS 组织如何与啮齿动物和人类的组织相关，目前还不清楚。在这里，我们通过电子显微镜和细胞类型特异性标志物的免疫组织化学检测，研究了成年和新生普通狨猴（Callithrix jacchus）的 SVZ 和 RMS，普通狨猴是一种广泛应用于神经科学的新世界灵长类动物。狨猴 SVZ 中的细胞在其标志物表达和形态上类似于啮齿动物 SVZ 的 B、C 和 A 型细胞。成年狨猴 SVZ 具有与人类大脑相似的三层结构，包括室管膜、细胞稀少和星形胶质细胞带层。然而，在成年-前 SVZ 和新生 SVZ 中，细胞稀少层非常薄或不存在。对成年狨猴 SVZ 前区的全室壁准备进行 PSA-NCAM 免疫染色，显示出广泛的拉长细胞聚集网络，类似于啮齿动物的神经母细胞链。在 Matrigel 中培养的狨猴 SVZ 外植体的延时记录显示，神经母细胞呈链状迁移，类似于啮齿动物的 A 型细胞。这些结果表明，SVZ 中神经发生和神经元迁移的一些特征在狨猴、人类和啮齿动物中是共同的。对成年狨猴 SVZ 的这一基本描述将有助于未来对灵长类动物成年神经发生的研究。
