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Studies on factors affecting release of gonadotrophins during the superfusion of isolated rat pituitaries.

作者信息

Kilpatrick M J, Newton J R, Collins W P

出版信息

J Reprod Fertil. 1977 Mar;49(2):347-51. doi: 10.1530/jrf.0.0490347.

DOI:10.1530/jrf.0.0490347
PMID:191605
Abstract

Isolated pituitary glands from adult male rats were maintained in a continuous flow system. Gn-RH (1000 pmol/ml) caused a characteristic release of cyclic AMP, LH, and FSH. Cyclic AMP (1000 nmol/ml) liberated a similar amount of both gonadotrophins. Theophylline (1 mmol/ml) enhanced the effect of cyclic AMP by 21% for LH and 41% for FSH. The infusion of oestradiol (184 pmol/ml) alone or before Gn-RH infusion did not produce a significant effect on the secretion of either gonadotrophin or cyclic AMP. In contrast, there was a significant reduction in the amount of LH (P less than 0-025) and FSH (P less than 0-05) released by pituitaries infused with oestradiol and cyclic AMP.

摘要

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