Wang Qiang, Wu Mei-Qing, Zhao Ling-Hui, Yang Hui-Ke, Lv Xiao-Hong
Inner Mongolia Forestry General Hospital, Yakeshi 022150, China.
Zhongguo Zhong Yao Za Zhi. 2008 Sep;33(18):2116-9.
To discuss the anti-tumor activity of ginsenoside Rh2, we observed the expressions of the junction adhesion molecul (JAM) in transplanted-tumor in mice.
The models of 40 transplanted-tumor mice that were established by subsequently injecting cancer ascite of mice (S180) with 0.2 mL per mouse into the preepipodite skin were divided into two groups. Experiment group was drenched with 2 mL ginsenoside Rh2 per mouse, equating to a dose 20 mg x kg(-1). Control group was drenched with 2 mL normal saline per mouse. The expression of JAM-1, JAM-2 in the lymphatics, blood vessels and tumours were observed by immunohistochemical staining.
The expression of JAM-1 on the cancer cells was significantly decreased in experiment group (IA 340.55) as compared with control group (IA 549.90, P<0.05). However, JAM-2 weakly expressed in both two groups. The density of blood vessels in which JAM-1, JAM-2 expressed showed 2.33 and 1.34 in control group, and 1.09 and 0.9 in experiment group respectively. Moreove, the density of lymph vessels were respectively 2.23 and 1.88 in control group compared with 0.99 and 0.79 in experiment group. The expression in blood vessels and lymph vessels in control group were significantly higher than those in experiment group, respectively (P<0.05).
Ginsenoside Rh2 can affect the tumor growth, further angiogenesis and lymphangiogenesis by down-regulating JAM expression in tumor.
为探讨人参皂苷Rh2的抗肿瘤活性，我们观察了小鼠移植瘤中连接黏附分子（JAM）的表达情况。
将40只通过向小鼠前足背皮肤注射0.2 mL小鼠癌腹水（S180）建立移植瘤模型的小鼠分为两组。实验组每只小鼠灌胃2 mL人参皂苷Rh2，相当于剂量20 mg·kg⁻¹。对照组每只小鼠灌胃2 mL生理盐水。通过免疫组织化学染色观察淋巴管、血管和肿瘤中JAM-1、JAM-2的表达。
与对照组（IA 549.90）相比，实验组癌细胞上JAM-1的表达显著降低（IA 340.55，P<0.05）。然而，JAM-2在两组中均弱表达。JAM-1、JAM-2表达的血管密度在对照组分别为2.33和1.34，在实验组分别为1.09和0.9。此外，对照组淋巴管密度分别为2.23和1.88，实验组分别为0.99和0.79。对照组血管和淋巴管中的表达分别显著高于实验组（P<0.05）。
人参皂苷Rh2可通过下调肿瘤中JAM的表达来影响肿瘤生长、进一步的血管生成和淋巴管生成。
