Wightman B, Bürglin T R, Gatto J, Arasu P, Ruvkun G
Department of Molecular Biology, Massachusetts General Hospital, Boston.
Genes Dev. 1991 Oct;5(10):1813-24. doi: 10.1101/gad.5.10.1813.
The heterchronic gene lin-14 controls the temporal sequence of developmental events in the Caenorhabditis elegans postembryonic cell lineage. It encodes a nuclear protein that normally is present in most somatic cells of late embryos and L1 larvae but is absent at later stages. Two lin-14 gain-of-function mutations delete 3'-untranslated sequences causing an inappropriately high level of the lin-14 nuclear protein late in development. These mutations identify a negative regulatory element that controls the formation of the lin-14 protein temporal gradient. The 21-kb lin-14 gene is differentially spliced to generate three lin-14 transcripts that encode protein products with variable amino-terminal regions and a constant carboxy-terminal region. The sequence of the gene revealed no protein sequence similarity to any proteins in various data bases.
异时基因lin-14控制秀丽隐杆线虫胚胎后细胞谱系中发育事件的时间顺序。它编码一种核蛋白，该蛋白通常存在于晚期胚胎和L1幼虫的大多数体细胞中，但在后期阶段不存在。两个lin-14功能获得性突变删除了3'非翻译序列，导致发育后期lin-14核蛋白水平异常升高。这些突变确定了一个控制lin-14蛋白时间梯度形成的负调控元件。21kb的lin-14基因进行差异剪接，产生三种lin-14转录本，它们编码具有可变氨基末端区域和恒定羧基末端区域的蛋白质产物。该基因的序列与各种数据库中的任何蛋白质均无蛋白质序列相似性。
