Graw J, Coban L, Liebstein A, Werner T
GSF-Institut für Säugetiergenetik, Neuherberg, F.R.G.
Gene. 1991 Aug 15;104(2):265-70. doi: 10.1016/0378-1119(91)90260-i.
The murine gamma E-crystallin-encoding gene (gamma E-cry) was isolated from a genomic DNA library. The nucleotide (nt) sequence was determined of 1100 bp upstream from the first exon to the polyadenylation site, comprising more than 3600 bp. The gene was characterized by phylogenetic nt sequence analysis in context with the already described gamma-cry genes from rat, mouse and human. The gamma E-cry genes (mouse and rat) are clearly separate from the corresponding gamma F-cry genes. Based on the phylogeny, the discussion about the murine gamma 2-cry classification as gamma F-cry [Bloemendal et al., Exp. Eye Res. 48 (1989) 465-466] is resolved. The murine gamma E-cry gene has characteristics similar to other genes from the gamma-cry gene family, except for an 18-fold repeat of the sequence, 5'-CTCAG, located at the 3'-end of intron B. There is no similar repeat structure in any other gamma-cry gene. No binding site for a common transcription factor could be detected among the 1100 bp of the 5'-region.
从小鼠基因组DNA文库中分离出了编码γE-晶状体蛋白的基因（γE-cry）。测定了从第一个外显子上游1100 bp到多聚腺苷酸化位点的核苷酸（nt）序列，该序列长度超过3600 bp。结合已报道的大鼠、小鼠和人类的γ-晶状体蛋白基因，通过系统发育核苷酸序列分析对该基因进行了表征。γE-晶状体蛋白基因（小鼠和大鼠）与相应的γF-晶状体蛋白基因明显不同。基于系统发育分析，解决了关于小鼠γ2-晶状体蛋白分类为γF-晶状体蛋白的争议[Bloemendal等人，《实验眼研究》48（1989）465 - 466]。小鼠γE-晶状体蛋白基因具有与γ-晶状体蛋白基因家族其他基因相似的特征，但内含子B的3'端存在一个18倍重复序列5'-CTCAG，这在其他任何γ-晶状体蛋白基因中都没有类似的重复结构。在5'区域的1100 bp中未检测到常见转录因子的结合位点。
