Chen Wei-liang, Ouyang Ke-xiong, Li Hai-gang, Huang Zhi-quan, Li Jing-son, Wang Jian-guang
Department of Oral and Maxillofacial Surgery, Center of Cranio-Maxillofacial Surgery, The Second Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
J Craniofac Surg. 2009 Jan;20(1):171-5; discussion 176-7. doi: 10.1097/SCS.0b013e31818435cd.
The purposes of this study were to determine the correlation between the expression of inducible nitric oxide synthase (iNOS) and vascular endothelial growth factor (VEGF) in ameloblastoma and to examine the relationships of this expression to angiogenesis and the clinical and biological behaviors of the tumor. Immunohistochemical staining with streptavidin peroxidase was used to analyze iNOS and VEGF expression, and CD34 was used to evaluate microvascular density (MVD) in 35 ameloblastomas (24 primary tumors and 11 recurrences) and 5 malignant ameloblastomas. Ten odontogenic keratocysts (OKCs) served as controls. On relational analysis, positive and VEGF expression and MVD counts increased in this order: OKCs, primary ameloblastoma, recurrent ameloblastoma, and malignant ameloblastoma. Differences between the ameloblastomas and OKCs were significant (P < 0.05). Among ameloblastomas, MVD counts increased with increasing expression of iNOS and VEGF (P < 0.05), and iNOS expression and VEGF expression were positively correlated (r = 0.66, P < 0.05). Inducible nitric oxide synthase expression and VEGF expression may be closely related to the angiogenesis and invasive biological behavior of ameloblastomas.
本研究的目的是确定成釉细胞瘤中诱导型一氧化氮合酶（iNOS）和血管内皮生长因子（VEGF）表达之间的相关性，并研究这种表达与血管生成以及肿瘤临床和生物学行为之间的关系。采用链霉抗生物素蛋白过氧化物酶免疫组织化学染色分析iNOS和VEGF的表达，并使用CD34评估35例成釉细胞瘤（24例原发性肿瘤和11例复发性肿瘤）和5例恶性成釉细胞瘤中的微血管密度（MVD）。10例牙源性角化囊肿（OKC）作为对照。相关性分析显示，VEGF表达阳性率和MVD计数按以下顺序增加：OKC、原发性成釉细胞瘤、复发性成釉细胞瘤和恶性成釉细胞瘤。成釉细胞瘤与OKC之间的差异具有统计学意义（P < 0.05）。在成釉细胞瘤中，MVD计数随iNOS和VEGF表达的增加而增加（P < 0.05），且iNOS表达与VEGF表达呈正相关（r = 0.66，P < 0.05）。诱导型一氧化氮合酶表达和VEGF表达可能与成釉细胞瘤的血管生成和侵袭性生物学行为密切相关。
