Gu Dayong, Shi Lei, Yu Huawei, Wang Hua, Lu Weiping, Liang Bing, Zhou Yuanguo, Zhang Ya'ou
Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Dec;25(6):1415-9.
The surface plasmon resonance (SPR)-based gene chip was prepared according to the following processes: First, a film of nanogold, which was synthesized by using Frens' method, was plated on chip by Chlorauric acid/hydroxylamine method. Then probes were fixed on nanogold film by Self-assembled monolayer (SAM) technology. Subsequently, the fixing time and concentration of probes, the sensitivity and the specificity of the chip were optimized. Our results suggested that the chip plated with 2.5 nm nanogold film has a better SPR reflection, and when fixed by probes for 4.5 h at the concentration of 1 500 nmol/L, the gene chip also shows a fine performance of detection and can identify accurately the mismatch between bases in SPR detection system. The gene chip constructed in the research can be used for SPR sensor detection.
基于表面等离子体共振（SPR）的基因芯片是按照以下步骤制备的：首先，采用弗伦斯法合成的纳米金薄膜通过氯金酸/羟胺法镀在芯片上。然后，通过自组装单分子层（SAM）技术将探针固定在纳米金薄膜上。随后，对探针的固定时间和浓度、芯片的灵敏度和特异性进行了优化。我们的结果表明，镀有2.5 nm纳米金薄膜的芯片具有更好的SPR反射，当在1500 nmol/L的浓度下用探针固定4.5小时时，该基因芯片在SPR检测系统中也表现出良好的检测性能，能够准确识别碱基错配。本研究构建的基因芯片可用于SPR传感器检测。
