Huang Yi-You, Hsu Hsin-Yun, Huang Chi-Jer Charles
Institute of Biomedical Engineering, College of Medicine, College of Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei, Taiwan.
Biosens Bioelectron. 2007 Jan 15;22(6):980-5. doi: 10.1016/j.bios.2006.04.017. Epub 2006 Jun 8.
Surface plasmon resonance (SPR) can detect molecules bound to a surface by subtle changes in the SPR angle. By immobilizing probes onto the surface and passing analyte solution through the surface, changes in SPR angle indicate the binding between analyte and probes. Detection of analyte from solution can be achieved easily. By using rolling circle amplification (RCA) and nanogold-modified tags, the signals of analyte binding are greatly amplified, and the sensitivity of this technique is significantly improved. Furthermore, this technique has potentials for ultra-sensitive detection and microarray analysis. In this paper, this detection technique is introduced and shown to have great amplification capability. Using 5 nm nanogold with 30 min of RCA development time, this proposed protein detection technique shows over 60 times amplification of the original signal.
表面等离子体共振（SPR）可以通过SPR角的细微变化来检测结合到表面的分子。通过将探针固定在表面上并使分析物溶液通过该表面，SPR角的变化表明分析物与探针之间的结合。从溶液中检测分析物可以很容易地实现。通过使用滚环扩增（RCA）和纳米金修饰的标签，分析物结合的信号被大大放大，并且该技术的灵敏度得到显著提高。此外，该技术具有超灵敏检测和微阵列分析的潜力。在本文中，介绍了这种检测技术并表明其具有强大的扩增能力。使用5纳米的纳米金并经过30分钟的RCA显影时间，这种提出的蛋白质检测技术显示出原始信号放大了60倍以上。
